槲皮素对2型糖尿病大鼠肾脏肥大的影响机制  

Influencing Mechanism of Quercetin on Kidney Hypertrophy in Type 2 Diabetic Rats

作　　者：王兴红[1] 常陆林[1] 王桂叶[1] 刘会君[1] 

机构地区：[1]漯河医学高等专科学校基础医学部,河南漯河462000

出　　处：《中国中医基础医学杂志》2015年第10期1248-1250,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：河南省教育厅高等学校重点科研项目(15B180011);漯河医学高等专科学校校级自然科学研究资助项目(2013-S-LMC24)

摘　　要：目的:观察槲皮素对2型糖尿病大鼠肾脏肥大的影响并探讨其作用机制。方法:建立2型糖尿病大鼠模型,按随机数字表法分为正常对照组、糖尿病模型组以及槲皮素组,测定各组大鼠血糖、血脂、总胆固醇、血清胰岛素、胰岛素敏感指数(ISI)、肾脏肥大指数和单根近端小管(PT)钠钾ATP酶(Na+,K+-ATPase)活性,免疫组化法测定肾脏Toll样受体4(TLR4)蛋白表达。结果:与模型组比较,槲皮素组大鼠血糖、血脂、总胆固醇、血清胰岛素水平、肾脏肥大指数、Na+,K+-ATPase活性和肾内TLR4蛋白表达显著降低,差异均有统计学意义。结论:槲皮素能抑制2型糖尿病大鼠肾脏肥大,其机制可能与降低血糖、血脂、总胆固醇和肾脏TLR4蛋白表达,升高ISI及稳定Na+,K+-ATPase活性有关。Objective： To observe the effect of quercetin on diabetic rats ＇ kidney hypertrophy and explore its mechanism. Methods： The model of type 2 diabetic rates was made. The rats were randomly divided into three groups：normal group,diabetic model group,and quercetin group. The blood sugar,blood fat,total cholesterol,serum insulin level,insulin sensitivity index（ ISI）,KW / BW,Na＋,K＋-ATPase activity of PT were measured. The toll-like receptor 4（ TLR4）protein expression was measured by immunohistochemical method. Results： Compared with the model group,The blood sugar,blood fat,total cholesterol,serum insulin level,KW / BW,Na＋,K＋-ATPase activity of PT,TLR4 protein expression were significantly reduced,so the differences were statistically significant. Conclusion： Quercetin can inhibit kidney hypertrophy,it may be caused by the decrease of blood sugar,blood fat,cholesterol,TLR4 protein expression and increase of ISI and stabilization of Na＋,K＋-ATPase activity of PT in T2 DM rats.

关 键 词：2型糖尿病 槲皮素 肾脏肥大 TOLL样受体4 钠钾ATP酶 

分 类 号：R285.5[医药卫生—中药学]