牛大力根油脂抗氧化活性研究  

Antioxidant Activity of Lipids from Millettia speciosa Root

作　　者：弓宝[1] 陈德力[1] 刘洋洋[1] 方草[2] 王德立[1] 杨新全[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所海南分所,海南万宁571533 [2]海南省农垦总医院,海南海口570311

出　　处：《中国现代中药》2015年第10期1033-1036,共4页Modern Chinese Medicine

基　　金：中央级公益性科研院所基本科研业务费专项(2013HNB02);海南省自然科学基金(20152019)

摘　　要：目的:研究牛大力根油脂抗氧化活性。方法:将牛大力根干燥粉末用70%乙醇浸提,结合硅胶柱层析分离,获得牛大力根油脂提取物F-1和F-2,同时采用加热回流法提取获得牛大力根油脂提取物F-3,并以DPPH法和FRAP法对各油脂提取物的抗氧化活性进行评价。结果:在DPPH法中,F-1、F-2及F-3的半数最高抑制浓度(IC50)值分别为203.18、138.38、244.10μg·m L-1,其FRAP值分别为(7 712.2±274.7)、(10 387.5±280.5)、(4 584.1±182.3)μmol Fe2+·g-1。抗坏血酸(Vc)和BHT的IC50值分别为11.59、53.69μg·m L-1,FRAP值分别为(17 356.1±622.9)、(13 235.4±469.5)μmol Fe2+·g-1。结论:牛大力根油脂具有一定的抗氧化活性,且以F-2最强。Objective：The aim of this study was to investigate the antioxidant activities of lipids from the Millettia speciosa root.Methods：The lipophilic extracts F-1 and F-2 were isolated from the dry root of M.speciosa by 70% ethanol extraction method and silica column chromatography.Meanwhile，lipophilic extract F-3 was extracted by heat reflux.Whereafter the antioxidant activity of F-1，F-2 and F-3 extracts were investigated by means of DPPH and FRAP assay.Results：The results showed that the half inhibitory concentration （IC50） of extracts of F-1，F-2 and F-3 were 203.18，138.38 and 244.10 μg·mL-1，respectively，with DPPH method，and their FRAP values were （7 712.2±274.7），（10 387.5±280.5） and （4 584.1±182.3） μmol Fe2＋·g-1，respectively，as well.The IC50 of ascorbic acid （Vc） and BHT were respectively 11.59 and 53.69 μg·mL-1，and their FRAP values were （17 356.1±622.9），（13 235.4±469.5） μmol Fe2＋·g-1.Conclusion：The oil fractions are moderate to good in scavenging effect on DPPH radical and reducing power.

关 键 词：牛大力 油脂 抗氧化 

分 类 号：R282.71[医药卫生—中药学]