激活处理对人卵子和早期胚胎ATP含量影响的初步研究  

Investigation of the ATP Content in Human Unfertilized Oocytes and Early-stage Embryos after Activation

作　　者：罗阳[1] 李莉[1] 孙源[1] 彭南妮[2] 刘见桥[1] 

机构地区：[1]广州医科大学附属第三医院生殖医学中心广东省生殖医学重点实验室,广东广州510150 [2]深圳市第五人民医院生殖医学中心,广东深圳518000

出　　处：《现代生物医学进展》2015年第30期5839-5842,共4页Progress in Modern Biomedicine

基　　金：广东省自然科学基金项目(S2012010008363)

摘　　要：目的:探讨激活处理对人未受精卵子和早期胚胎三磷酸腺苷(ATP)含量影响。方法:收集辅助生殖治疗周期中体外受精(IVF)以及单精子卵母细胞浆内注射(ICSI)时的未受精卵子(包括GV期、MⅠ期、MⅡ期卵母细胞),以及早期胚胎(2-cell、4-cell胚胎),采用生物化学发光法测定在未处理和进行激活处理后的ATP含量。结果:1未处理的GV期、MⅠ期、MⅡ期卵子和2-cell、4-cell期胚胎的ATP含量分别为1.14±0.15、1.41±0.14、1.45±0.12、1.61±0.25、2.05±0.23 pmol,激活处理后GV期、MⅠ期、MⅡ期卵子和2-cell、4-cell期胚胎的ATP含量分别为1.60±0.17、1.86±0.25、2.06±0.38、2.26±0.53、2.55±0.46 pmol;2激活处理后GV期、MⅠ期卵子的ATP含量显著性高于未处理组(P<0.05)。结论:人GV期、MⅠ期、MⅡ期卵子和2-cell、4-cell期胚胎中ATP含量逐渐增加;经激活处理后,仅GV期、MⅠ期卵子的ATP含量有显著性变化。Objective： To investigate the impact of activation to the ATP content in human unfertilized oocytes and early-stage embryos. Methods： The unfertilized oocytes（include germinal vesicle, metaphase I, metaphase II oocytes） and early-stage embryos（include 2-cell, 4-cell embryos） from IVF and ICSI in assisted reproduction cycles, were detected by ATP-bioluminescence assay to analyzed the ATP content before and after activation. Results： 1 The ATP content of human GV, MI, MII oocytes and 2-cell,4-cell embryos in untreated and activated group were 1.14 ±0.15, 1.41 ±0.14, 1.45 ±0.12,1.61 ±0.25, 2.05 ±0.23 pmol, and 1.60 ±0.17,1.86±0.25, 2.06±0.38, 2.26±0.53, 2.55±0.46 pmol. 2The ATP content of GV and MI oocytes in activated group was significantly higher than that in untreated group（P〈0.05）. Conclusion： The ATP content of human GV, MI, MII oocytes and 2-cell, 4-cell embryos is keep increasing; Only the ATP content of GV and MI oocytes after activation treatment were significantly higher than the untreated group.

关 键 词：未受精卵子 早期胚胎 卵子激活 ATP含量 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]