土壤矿质元素与当归HPLC指纹图谱共有峰的关联分析  

Correlation Analysis Between Common Peaks of Angelica sinensis HPLC Fingerprint and Mineral Elements in Its Growing Soil

作　　者：丁军霞 顾志荣[1] 王亚丽[1,2] 张亚亚[1] 王耀鹏[1] 孙宇靖[1,2] 

机构地区：[1]甘肃中医学院科研实验中心/甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000 [2]甘肃中医学院当归研究所,甘肃兰州730000

出　　处：《中药材》2015年第8期1595-1599,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(30960037);2011年甘肃省发改委战略新兴产业和产业技术研究与开发专项项目;甘肃中医学院研究生创新基金项目(CX2014-06);甘肃省科技厅科技支撑计划(144FKCA078)

摘　　要：目的:探讨当归产地土壤中矿质元素与当归HPLC指纹图谱共有峰的相关性。方法:获取12个产地120批当归药材的HPLC指纹图谱,以电感耦合等离子体质谱(ICP-MS)或原子吸收光谱(AAS)测定相应的产地土壤中15种矿质元素含量,采用双变量相关和多元线性回归研究两者的相关性。结果:土壤中多种矿质元素与当归HPLC指纹共有峰的多个共有峰同时存在显著(P<0.05)或极显著(P<0.01)的正相关或负相关;土壤矿质元素对峰1的贡献大小为Zn>K>Sb>Fe>Na,对峰6(正丁基苯酞)的贡献大小为Mn>Mg>Ca,对峰7的贡献大小为Cr>Zn,对峰8的贡献大小为Mn>Na,对峰11的贡献大小为As>K>Fe>Cd,对峰12的贡献大小为Zn>Mn>K,对峰13(正丁烯基苯酞)的贡献大小为Mn>Zn>Cd,对峰15的贡献大小为Zn>K,对峰16的贡献大小为Fe>Ni,对峰17的贡献大小为Zn>Mn>Ni>Fe>K,对峰18的贡献大小为Zn>Na;峰2、3(阿魏酸)、4及14(Z-藁本内酯)分别主要受土壤中As、Zn、Sb及Cu的影响。结论:土壤矿质元素与当归HPLC指纹共有峰的关系具有复杂性、多重性及交互性,在土壤微肥施放及当归种植中应选择性地加以考察。Objective：To investigate the correlation between common peaks of Angelica sinensis HPLC fingerprint and mineral ele- ments in its growing soil. Methods ：The fingerprints of 120 batches of Angelica sinensis from 12 habitats were determined by HPLC. The contents of Pb, As, Cr, Sb, Hg, Cu, Cd, Ni, Zn, Mg, Mn, Ca, Fe, Na and K in corresponding soil were determined by ICP-MS and AAS. Bivariate and multiple linear regression were used to analyze the correlation. Results ： There were significant （ P 〈 0. O1 or P 〈 0. 05 ） positive and negative correlation between many common peaks in HPLC fingerprint of Angelica sinensis and mineral elements in its growing soil. The contribution of mineral elements in soil on peak 1 were Zn 〉 K 〉 Sb 〉 Fe 〉 Na; on peak 6 （3-butylphthalide） were Mn 〉 Mg 〉 Ca;on peak 7 were Cr 〉 Zn;on peak 8 were Mn 〉 Na;on peak 11 were As 〉 K 〉 Fe 〉 Cd;on peak 12 were Zn 〉 Mn 〉 K;on peak 13 （Z-butylidenephthalide） were Mn 〉 Zn 〉 Cd ; on peak 15 were Zn 〉 K; on peak 16 were Fe 〉 Ni ; on peak 17 were Zn 〉 Mn 〉 Ni 〉 Fe 〉 K;on peak 18 were Zn 〉 Na;peaks 2,3 （ferulic acid） ,4 and 14 （Z-ligustilide）was mainly affected by As, Zn, Sb and Cu, re- spectively. Conclusion：The relationship between HPLC fingerprint peak of Angelica sinensis and mineral elements in its growing soil shows complexity,multiplicity and interactivity,which should be selectively examined during manuring micronutrient fertilizer and Angelica sinensis cultivation.

关 键 词：土壤矿质元素 当归 HPLC指纹图谱 双变量相关分析 多元线性回归 

分 类 号：R282.2[医药卫生—中药学]