柱前衍生化HPCE法测定不同种源石斛中多糖的单糖组成  

Study on Monosaccharide Compositions of Polysaccharide in Dendrobium Stems of Different Resources by PMP-HPCE

作　　者：陈乃东[1,2,3] 孟云飞[1] 姚厚军[1] 曹彩芸 陈辰[1] 李俊[3] 

机构地区：[1]皖西学院生物与制药工程学院,安徽六安237012 [2]皖西中药与天然药物工程技术研究中心,安徽六安237012 [3]安徽医科大学药学院,安徽合肥230032

出　　处：《中药材》2015年第8期1607-1610,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81274021);中国博士后研究面上项目(2014M551791);安徽省人事厅博士后研究项目;安徽高校省级科学研究重点项目(KJ2014A279);皖西学院本科生优秀毕业设计(论文)培育计划立项项目(pyjh2014061);皖西学院学生研究性学习项目(wxxyx2014124;wxxyx2014126)

摘　　要：目的:采用PMP(1-苯基-3-甲基-5-吡唑啉酮)柱前衍生化-HPCE法测定多糖的单糖组成,探讨不同种源霍山石斛、铜皮石斛及野生河南石斛药效物质基础的相似性。方法:采用石英毛细管柱(100 cm×50μm),350mmol/L硼酸溶液(以1.0 mol/L Na OH溶液调至p H 10)为缓冲液,运行电压20 k V,柱温为25℃,检测波长250 nm,运行时间34 min。结果:5种石斛样品共检出木糖、葡萄糖、甘露糖、半乳糖、半乳糖醛酸和核糖6种单糖。相似度评价显示,同一种源石斛样品10个批次间的相似度均在0.98以上,但不同种源的霍山石斛、铜皮石斛及野生河南石斛单糖组成及含量明显不同。结论:多糖部位PMP柱前衍生化-HPCE法具有简单、快速、准确、重现性好等优点,为不同种源的霍山石斛、铜皮石斛与野生河南石斛的定性鉴别及药材内在质量评价提供新的参考依据。Objective：To establish a PMP-HPCE method for comparing the monosaccharides of polysaccharide in tissue-cultured and wild Dedrobium huoshanese and Dedrobium moniliforme as well as wild Dedrobium henanese, in order to investigate the similarities of their bioaetive components. Methods：The PMP-monosaccharides of polysaccharide from the five investigated Dedrobium samples were separated by HPCE on a fused silica capillary column（ 100 cm × 50μm）at 25℃ with 350 mmol/L BAS（ adjusted to pH 10 with 1.0 mol/L NaOH） as running buffer for 34 min. The applied voltage was 20 kV and the detection wavelength was set at 250 nm. Results ：Total six monosaceharides including xylose, glucose, mannose, galaetose, galacturonic acid and ribose were detected in the five Dendrobiurns samples and the similarity coefficients between the ten batches of the same Dendrobium species were all above 0. 98 ,while remarkable dissimilarity were exhibited among species and different resources. Conelusion：PMP-HPCE technique combined with chemometrics is simple, convenient, precise, reproducible and proved to be an effective strategy for identifying the species and origins, especially in the quality assessment of Dendrobium stems.

关 键 词：霍山石斛 铜皮石斛 野生河南石斛 高效毛细管电泳 多糖 

分 类 号：R284.1[医药卫生—中药学]