正交试验法优选左归丸的醇提和水提工艺  

Optimization of Ethanol Extraction and Water Extraction Technologies of Zuogui Pills by Orthogonal Test

作　　者：李玲[1] 王冰[1] 张彤[1] 徐本亮[1] 袁秀荣[1] 

机构地区：[1]上海中医药大学,上海201203

出　　处：《中药材》2015年第8期1724-1727,共4页Journal of Chinese Medicinal Materials

基　　金：新世纪优秀人才支持计划(NCET-10-0944);上海市教委知识服务平台项目

摘　　要：目的:优选左归丸处方最优醇提和水提工艺。方法:采用正交试验法,考察乙醇浓度、加醇量、回流时间对菟丝子中金丝桃苷转移率、山茱萸中熊果酸转移率及干膏得率的影响,考察浸泡时间、加水量、提取时间对熟地黄中毛蕊花糖苷转移率及干膏得率的影响,优化得到左归丸的醇溶和水溶性成分的最优提取工艺。结果:最佳醇提工艺为:取菟丝子、山茱萸和牛膝药材,加8倍量70%乙醇回流提取2次,每次1 h;最佳水提工艺为:醇提后药渣与其余药材加12倍量水,浸泡0.5 h,煎煮2次,每次0.5 h。结论:所确定的提取工艺对醇溶和水溶性有效成分提取率高、工艺稳定可行,为左归丸的二次开发提供了参考依据。Objective：To optimize the ethanol extraction and water extraction technologies of Zuogui Pills. Methods：An orthogonal test was used to study the influence of ethanol concentration, amount of ethanol and reflux time on hyperoside in Cuscutae Semen, ursolic acid in Corni Fructus and the yield of dry paste. Also, The effect of soaking time, amount of water and extraction time on acteoside in Rehmanniae Radix Praeparata and the yield of dry paste were investigated. All these factors were adopted as the indices for choosing the best ethanol extraction and water extraction technologies. Results： The best ethanol extraction technology for Cuscutae Semen, Corni Fructus and Achyranthis Bidentatae Radix was reflux extracting for 1 h each time for two times with eight times of 70% ethanol. The best water extraction technology for the residue and the rest herbs after the ethanol extraction was soaking for 0. 5 h and then decoeting for 0. 5 h each time for two times with twelve times of water. Conclusion：The optimal extraction technologies are stable and feasible,the extraction rate of the alcohol soluble and water soluble effective components is high, and it provides the reference basis for the secondary development of Zuogui Pills.

关 键 词：左归丸 正交试验 提取工艺 金丝桃苷 熊果酸 毛蕊花糖苷 

分 类 号：R286.2[医药卫生—中药学]