HPLC法在测定番泻总苷胶囊中番泻苷AB含量中的应用  

Application of HPLC method for determination of sennoside A and B contents in Senna Total Glycosides Capsules

作　　者：张苗[1] 顾燕 

机构地区：[1]江苏省中医院,江苏南京210029 [2]江苏睿博医药有限公司,江苏南京211112

出　　处：《现代医药卫生》2015年第21期3235-3237,共3页Journal of Modern Medicine & Health

摘　　要：目的建立番泻总苷胶囊中番泻苷A、B含量的测定方法。方法采用高效液相色谱（HPLC）法,以八烷基硅烷键合硅胶柱、乙腈-0.1%三氟乙酸水溶液为流动相梯度洗脱,检测波长340 nm。结果番泻苷A在0.210 4~1.052 0μg、番泻苷B在0.225 0~1.125 0μg呈良好的线性关系,平均回收率分别为99.51%（RSD=1.30%）和99.07%（RSD=0.69%）。结论该方法简单快捷、结果可靠,可作为番泻总苷胶囊质量控制的方法。Objective To establish a method for the determination of sennoside A and B contents in Senna Total Glyco-sides Capsule. Methods The high performance liquid chromatography（HPLC） method was adopted,the gradient elution was performed with octadecyl silane bonded silica gel column,the mobile phase of acetonitrile-0.1% trifluoroacetic acid aqueous solution and the detection wavelength of 340 nm. Results Sennoside A in the range of 0.210 4-1.052 0 μg and sennoside B in the range of 0.225 0-1.125 0 μg had good linear relationship. The average recovery rates were 99.51%（RSD=1.30%） for sennoside A and 99.07%（RSD =0.69%） for sennoside B. Conclusion The established method is simple,quick and reliable,which can be used as a quality control method for Senna Total Glycosides Capsules.

关 键 词：番泻叶(中药) 胶囊 番泻甙/分析 色谱法 高压液相 

分 类 号：R284.1[医药卫生—中药学]