PD －L1及 CD86在脓毒症小鼠外周血单核细胞中的表达趋势  

Expression of PD -L1 and CD86 in the peripheral blood mononuclear cells of septic mice

作　　者：张小玲[1] 王国恩[1] 张军[2] 刘媛媛[1] 刘敏龙[1] 

机构地区：[1]西安交通大学医学院第二附属医院重症医学科,陕西 西安710004 [2]西安交通大学医学院第二附属医院消化内科,陕西 西安710004

出　　处：《中国急救医学》2015年第10期950-953,I0003,共5页Chinese Journal of Critical Care Medicine

基　　金：陕西省2014年度科学技术研究发展计划项目

摘　　要：目的：观察脓毒症小鼠外周血单核细胞负性共刺激分子PD－L1及正性共刺激分子CD86的表达趋势，探讨脓毒症小鼠的免疫状态。方法60只C57小鼠随机分为6 h、24 h、48 h脓毒症模型组及假手术组，每组10只小鼠。采用盲肠结扎穿孔法造模，造模成功后分别于6 h、24 h、48 h取外周血，用流式细胞仪检测各组单核细胞PD－L1及CD86的表达，同时对小肠进行HE染色观察形态学变化。结果在100＆#215;光镜下观察24 h及48 h模型组小鼠小肠上皮黏膜绒毛破坏严重，萎缩明显，假手术组和6h模型组小肠黏膜绒毛正常。模型组6h、24h、48h单核细胞PD－L1及CD86的表达均较假手术组升高（P均＜0．05），且PD－L1于24 h、48 h升高更明显（P＜0．01）。模型组中，PD－L1及CD86均在6 h开始升高，24 h达峰值，48 h开始下降。结论脓毒症小鼠外周血PD－L1及CD86表达均升高，且PD－L1升高更明显，提示脓毒症早期机体特异性免疫主要呈现负性调节。Objective To observe the expression trend of negative costimulatory molecule programmed death ligand 1 （ PD -L1 ） and positive costimulatory molecule CD 86 in peripheral blood mononuclear cells of septic mice , to investigate the immune status of sepsis in mice .Methods Sixty C57 mice were randomly divided into 6 h, 24 h, 48 h sepsis model group and the sham group , 10 mice in each group .Sepsis model was reproduced by cecal ligation and puncture .6 h, 24 h, 48 h after successful modeling peripheral blood mononuclear cells were taken for detection of PD -L1 and CD86 by flow cytometry, while HE staining was used to observe the morphological changes of intestine .Results Under the 100 ＆#215; light microscope , the 24 h and 48 h sepsis group showed serious intestinal epithelial mucosal villous damage , but the sham group and 6 h sepsis group showed normal small intestinal villi . CD86 and PD-L1 expression in sepsis model group in 6 h, 24 h, 48 h was all increased compared with that in sham group （P〈0.05）.And elevated levels of PD-L1 in 24 h, 48 h is more significant （P〈0.01）.CD86 and PD-L1 were increased at the beginning of 6 h, 24 h reached the peak, and 48 h began to decline .Conclusion Peripheral blood mononuclear cells in sepsis mice showed increased expression of CD86 and PD -L1, and the rise of PD -L1 level was even more significant , which suggests that negative regulation of specific immunity is dominant during the early stage of sepsis .

关 键 词：脓毒症 程序性细胞死亡因子配体1(PD-L1) CD86 单核细胞 

分 类 号：R512.62[医药卫生—内科学]