KM突变小鼠皮肤T、B细胞数量分布和皮肤细胞凋亡增殖的动态改变  

Dynamic Changes of the Quantitative Distribution,Apoptosis and Proliferation of T and B Cells in the Skin of KM Mutant Mice

作　　者：李彦红[1] 刘颖[1] 黄澜[1] 徐艳峰[1] 朱华[1] 李涛[2] 邓巍[1] 秦川[1] 

机构地区：[1]中国医学科学院北京协和医学院医学实验动物研究所卫生部人类疾病比较医学重点实验室国家中医药管理局人类疾病动物模型三级实验室,北京100021 [2]中国医学科学院北京协和医学院医务室,北京100730

出　　处：《中国医学科学院学报》2015年第5期489-495,共7页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金青年基金(31301928)~~

摘　　要：目的观察不同年龄段KM突变小鼠皮肤T、B淋巴细胞的数量和分布,表皮及毛囊皮脂腺细胞凋亡和增殖情况。方法采用血常规、免疫组织化学检测KM突变小鼠皮肤T、B淋巴细胞改变情况,TUNEL技术和增殖细胞核抗原（PCNA）染色检测1 d、3 d、6 d、9 d、22 d、3个月、6个月KM突变小鼠皮肤组织背皮细胞凋亡和增殖情况。结果 KM突变小鼠皮肤T细胞主要表达在表皮及真皮,出生第1天至第6天增多,9 d、22 d减少,3个月以后增多,6个月时可见B细胞增多。KM突变小鼠凋亡细胞主要分布在毛囊和皮脂腺,较野生小鼠增多,且两种小鼠均在22 d时凋亡细胞数目最多;出生1～9 d的KM突变小鼠皮肤表皮基底细胞增殖与野生小鼠无差异,22 d和3个月增殖减少,但6个月时增殖多;毛囊及皮脂腺细胞22 d时增殖较多,9 d和3月龄增殖减少。结论 KM自发突变小鼠皮肤组织存在T、B淋巴细胞数量分布及毛囊皮脂腺细胞凋亡和增殖的异常改变,引起免疫学紊乱和被毛异常生长,促进了炎症反应。Objective To observe the change of quantitative distribution,apoptosis and proliferation of T and B cells in the skin of KM mutant mice. Methods We chose 1-,3-,6-,9-,22-day,3-,6-month-old KM mutant and wild-type mice to detect the changes of T and B lymphocytes using blood routine tests and immu-nohistochemical staining. Apoptosis was detected by TUNEL staining and proliferation by proliferating cell nuclear antigen（ PCNA） staining. Results T cells on KM mutant mice skin were mainly seen in epidermis and dermis.They increased on the first day to 6^th day after birth and decreased on the 9^th and 22^nd day,but after 3-monthold,their number began to increase; at the time of 6 months,the number of B cells also increased. The apoptosis of the skin hair follicle and sebaceous gland cells were more obvious in KM mutant mice than in wild-type mice,with the maximal apoptosis occurred at the age of 22-day-old in both groups. The proliferation of epidermal basal cells in KM mutant mice between 1 to 9-day-old was not significantly different from that in the wild-type mice,but decreasing on the 22^nd day and 3^rd month and increasing in the 6thmonth. The proliferation in hair follicle and sebaceous glands decreased on 9^th day,increased on 22^nd day,and deceased on the 3^rd month again.Conclusions The quantitative distribution,apoptosis,and proliferation of T and B lymphocytes abnormally change in the skin tissue of KM spontaneous mutant mice. They may lead to immune and hair growth disorders and promote the inflammatory responses.

关 键 词：KM小鼠 自发突变 T淋巴细胞 B淋巴细胞 凋亡 增殖 

分 类 号：R392.3[医药卫生—免疫学]