佑福泉水对2型糖尿病模型大鼠血糖的影响  

THE EFFECT OF YOUFUQUAN WATER ON TYPE 2DIABETIC RATS

作　　者：刘占涛[1] 杨志宏[1] 姜凌燕[1] 高华[1] 

机构地区：[1]青岛大学药学院,山东青岛266021

出　　处：《齐鲁医学杂志》2015年第6期677-680,共4页Medical Journal of Qilu

摘　　要：目的观察佑福泉水对实验性2型糖尿病大鼠血糖、胰岛素耐量和葡萄糖耐量的影响。方法雄性大鼠80只,采用高糖高脂饲料喂养5周加小剂量链脲佐菌素（40mg/kg）腹腔注射的方法建立实验性2型糖尿病大鼠模型,筛选后随机分为4组,每组10只。模型组给予蒸馏水10 mL/kg灌胃,二甲双胍组给予二甲双胍250mg/kg灌胃,佑福泉水高剂量组每天饮用佑福泉水并以10mL/kg灌胃,佑福泉水低剂量组每天用佑福泉水稀释1倍饮用并以10mL/kg灌胃,同时随机抽取10只大鼠作为对照组（每天以10mL/kg蒸馏水灌胃）,各组均每天灌胃1次,连续给药5周。在实验的第2、4、5周末测各组空腹血糖,于第4、5周末进行胰岛素耐量和葡萄糖耐量实验,实验期间观察大鼠的体质量及饮水量的变化。结果从给药第2周起,模型组大鼠体质量较对照组明显下降（F=2.79~9.67,q=3.67~7.89,P〈0.05）;给药后5周,二甲双胍组及佑福泉水高、低剂量组大鼠体质量较模型组有所增加,但差异无统计学意义（P〉0.05）。给药后3~5周,二甲双胍组和佑福泉水组,尤其是高剂量组大鼠饮水量较模型组明显减少（F=24.39~99.49,q=4.04~7.45,P〈0.05）。给药2周后,二甲双胍组及佑福泉水组血糖值均呈下降趋势,第5周末,二甲双胍组与模型组比较血糖下降明显（q=3.96,P〈0.05）;二甲双胍组注射胰岛素后血糖曲线下面积（AUC）与模型组比较下降明显（F=33.35,q=3.03,P〈0.05）,佑福泉水组AUC也低于模型组但差异无显著性（P〉0.05）;口服葡萄糖后,与模型组比较,二甲双胍组AUC明显下降（F=170.79,q=7.57,P〈0.05）,佑福泉水低剂量组AUC明显下降（q=3.83,P〈0.05）。结论佑福泉水对2型糖尿病模型大鼠有降低血糖趋势,能一定程度改善糖耐量异常和胰岛素抵抗作用。Objective To observe the effect of Youfuquan water on blood sugar,insulin tolerance and glucose tolerance in type-2-diabetic rat models. Methods Eighty male rats were fed with high-glucose and high-fat forage for five weeks,and then intraperitoneal injection of streptozotocin（40mg/kg）to create a rat model of type-2diabetes.After screening,the rats were randomized to four groups with 10 rats in each group.The rats in model group were given intragastric administration of 10mL/kg of distilled water;those in metformin group were given intragastric administration of metformin（250 mg/kg）;those in high-doseYoufuquan-water group drank the water every day and added intragastric administration of the water;those in low-dose-Youfuquan-water group drank double dilution of Youfuquan water and given intragastric administration of Youfuquan water（10mL/kg）.Ten rats were selected in random to serve as controls,intragastric administration of 10mL/kg of distilled water was given.Intragastric administration was given daily to rats in all the groups for five weeks.In the end of weeks 2,4and 5of the experiment,fasting blood sugar was tested in each group,in the end of weeks 4and 5,insulin tolerance test and glucose tolerance test were conducted,during the experiment,the changes of bodyweight of the rats and water intake were recorded. Results Starting from the second week of medication,the body weight of rats in the model group markedly declined as compared with that in the control group（F=2.79-9.67,q=3.67-7.89,P〈0.05）,and after five weeks,the body weight of rats in metformin,high-dose-and lowdose-Youfuquan groups increased somewhat as compared with the model group,but the differences were not statistically significant（P〉0.05）.After 3-5-week medication,the volume of water intake in rats in metformin and Youfuquan water groups,especially those in high-dose group,obviously reduced versus the model group（F=24.39-99.49,q=4.04-7.45,P〈0.05）.The blood sugar levels in rats of metformin and Youfuquan water groups

关 键 词：糖尿病 2型 佑福泉水 葡萄糖耐量试验 胰岛素抗药性 

分 类 号：R969.4[医药卫生—药理学]