三七皂苷R_1对D-半乳糖诱导痴呆模型小鼠学习记忆能力的影响及抗氧化应激作用研究  

Effect of Notoginsenoside R_1 on Learning and Memory Ability and Antioxidation Stress in the Dementia Mice Induced by D-galactose

作　　者：黄金兰[1] 秦嫦云 周楠[1] 高山[1] 杜伟[1] 谢艳秋[1] 王霞[1] 施倩[1] 吴登攀[1] 

机构地区：[1]徐州医学院药学院,江苏徐州221004

出　　处：《中国药房》2015年第31期4336-4338,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81460598);江苏省教育厅开放研究课题(No.KJS1404);江苏省新药与临床药学重点实验室开放研究课题(No.ZR-XY201402);徐州医学院2014年大学生实践创新训练计划推荐项目(No.201410313032Y);徐州医学院优秀人才科研启动基金项目(No.D2014017)

摘　　要：目的:研究三七皂苷R1(NGR1)对老年性痴呆模型(即AD模型)小鼠的学习记忆能力的影响及抗氧化应激作用。方法:将小鼠随机分为正常组、模型组、阳性对照(吡拉西坦,150 mg/kg)组和NGR1低、高剂量(12.5、25 mg/kg)组,每组10只。除正常组ih等容生理盐水外,其余各组每日于颈背部ih 10%D-半乳糖以复制AD小鼠模型,qd;并于造模同时ig相应药物,正常组和模型组ig蒸馏水,qd。4周后采用Morris水迷宫试验检测小鼠原平台象限游泳时间百分比;可见分光光度法检测小鼠血清、脑组织中总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX)和过氧化氢酶(CAT)活性;酶联免疫吸附法检测小鼠脑组织中8-羟基脱氧鸟苷(8-OHd G)含量。结果:与正常组比较,模型组小鼠的原平台象限游泳时间百分比明显缩短,血清和脑组织中T-SOD、GSH-PX和CAT活性明显降低,脑组织中8-OHd G含量则明显升高,差异均有统计学意义(P<0.01);与模型组比较,各给药组小鼠的原平台象限游泳时间百分比明显延长,血清和脑组织中T-SOD、GSH-PX、CAT活性均显著提高,脑组织中8-OHd G含量减少,差异均有统计学意义(P<0.01或P<0.05)。结论:NGR1可通过减轻氧化应激从而改善AD模型小鼠的学习记忆能力。OBJECTIVE：To study the effect of notoginsenoside R1（NGR1）on learning and memory ability and antioxidation stress in the senile dementia model（AD model） mice. METHODS：The mice were randomly divided into normal group,model group,positive control group（piracetam,150 mg/kg）,NGR1low-dose and high-dose groups（12.5,25 mg/kg）with 10 mice in each group. The mice were ih given 10% D-galactose on neck and back,once a day,to induce AD mice model except normal group was ih given constant volume of normal saline. At the same time,they were given relevant medicine intragastrically,and normal group and model group were given distilled water intragastrically,once a day. 4 weeks later,the percentage of swimming time in original platform quadrant was assayed by Morris water maze;the activities of T-SOD,GSH-PX and CAT in serum and cerebral tissue were detected by visible spectrophotometry;the content of 8-hydroxy-2-deoxyguanosine（8-OHd G）in cerebral tissue was tested by enzyme linked immunosorbent assay（ELISA）. RESULTS：Compared with normal group,the percentage of swimming time in original platform quadrant decreased in model group,and the activities of T-SOD,GSH-PX and CAT in serum and cerebral tissue decreased,while the content of 8-OHd G in cerebral tissue increased,with statistical significance（P〈0.01）;compared with model group,the percentage of swimming time in original platform quadrant increased in treatment groups,and the activities of T-SOD,GSH-PX and CAT in serum and cerebral tissue increased significantly,while the content of 8-OHd G in cerebral tissue decreased,with statistical significance（P〈0.01 or P〈0.05）. CONCLUSIONS：NGR1can improve the learning and memory ability of AD mice by means of alleviating the oxidative stress.

关 键 词：三七皂苷R1 D-半乳糖 老年性痴呆模型 学习记忆 抗氧化应激 小鼠 

分 类 号：R285.5[医药卫生—中药学] R969.3[医药卫生—中医学]