雷公藤甲素对类风湿关节炎患者滑膜成纤维样细胞增殖的体外抑制作用研究  

Study on Inhibitory Effects of Triptolide on the Proliferation of Fibroblast-like Synovial Cells from Patients with Rheumatoid Arthritis in vitro

作　　者：叶蕾[1] 贾霜[1] 潘文萍[2] 

机构地区：[1]山东省千佛山医院药学部,济南250014 [2]山东省千佛山医院风湿免疫科,济南250014

出　　处：《中国药房》2015年第31期4357-4359,共3页China Pharmacy

基　　金：山东省中医药科技发展计划项目(No.2011-191)

摘　　要：目的：研究雷公藤甲素（TP）对体外培养的类风湿关节炎（RA）患者成纤维样滑膜细胞（FLS）增殖的影响。方法：从5例RA患者的膝关节置换术或滑膜切除术中获得滑膜组织,经分离、培养、鉴定得到FLS后分别与0（空白对照）、50、100、200 nmol/ml TP共孵育24、48、72 h。采用MTT法检测TP对FLS增殖的影响,计算增殖抑制率;流式细胞仪检测细胞凋亡和细胞周期。结果：50、100、200 nmol/ml TP对FLS的增殖抑制率为17.46%~52.56%,且抑制作用与药物浓度呈正相关。与空白对照组比较,100、200 nmol/ml TP作用后G0/G1期细胞比例增加,S期细胞比例减少,差异有统计学意义（P〈0.05）;200 nmol/ml TP可诱导细胞凋亡。结论：TP对RA患者体外培养的FLS具有一定的增殖抑制作用并可诱导其凋亡,这可能是TP治疗RA的机制之一。OBJECTIVE：To investigate the effects of triptolide（TP）on the proliferation of fibroblast-like synoviocytes（FLS）from patients with rheumatoid arthritis（RA）in vitro. METHODS：5 RA patients received knee arthroplasty or synovectomy to obtain synovial tissue. FLS was isolated,cultured and identified,and then incubated in the presence of TP [0（blank control）,50,100 and 200 nmol/ml] for 24,48 and 72 h. The effects of TP on FLS was evaluated by MTT,and then proliferation inhibitory rate was calculated;flow cytometry was used to detect the apoptosis and cell cycle of FLS. RESULTS：The inhibitory rates of TP（50,100 and 200 nmol/ml）on the proliferation of FLS were 17.46%-52.56%,which was positively correlated with drug concentration.Compared with blank control group,100 and 200 nmol/ml TP could increase the percentage of cells in G0/G1 phase and decrease the percentage of cells in S phase,with statistical significance（P〈0.05）;200 nmol/ml TP could induce cell apoptosis. CONCLUSIONS：TP could inhibit the proliferation and also could induce the apoptosis of FLS in RA patients in vitro,which may be one of its mechanism for treating RA.

关 键 词：类风湿关节炎 滑膜成纤维细胞 雷公藤甲素 增殖 抑制 机制 

分 类 号：R285.5[医药卫生—中药学]