α-突触核蛋白在食蟹猴消化道不同部位的表达  

Expression of α-synuclein in different regions of digestive tract of cynomolgus monkeys

作　　者：李旭冉[1,2] 杨巍巍[1,2] 李昕[1,2] 于顺[1,2,3] 

机构地区：[1]首都医科大学宣武医院神经生物学研究室,北京100053 [2]帕金森病研究北京重点实验室,北京100053 [3]北京脑重大疾病研究院帕金森病研究所,北京100053

出　　处：《首都医科大学学报》2015年第5期675-679,共5页Journal of Capital Medical University

基　　金：国家重点基础研究发展计划(2011CB504101);国家自然科学基金(81071014;81371200;81401042);国家科技支撑计划课题(2012BAI10B03);首都卫生发展科研专项课题(2011-4001-01);北京市自然科学基金(7122035)~~

摘　　要：目的观察α-突触核蛋白(α-synuclein,α-Syn)在正常食蟹猴消化道不同部位的表达。方法利用免疫组织化学和Western blotting方法检测α-Syn在食蟹猴食道、胃、十二指肠、空肠、回肠及结肠中的表达特点和表达量。结果α-Syn免疫活性物质主要见于消化道壁内神经的神经纤维和神经节,这些α-Syn免疫阳性神经结构见于消化道的黏膜、黏膜下层和肌层。在消化道的不同部位,α-Syn的表达量不同,其中十二指肠、空肠、回肠和结肠表达量较高,而胃和食道的表达量较低。结论α-Syn主要存在于食蟹猴消化道的壁内神经,在消化道的不同部位表达量差异有统计学意义。Objective To investigate the expression of α-synuclein（ α-Syn） in different regions of the digestive tract of cynomolgus monkeys. Methods Immunohistochemistry and Western blotting analysis were applied to observe the expression of α-Syn in the digestive tract of cynomolgus monkeys. Results α-Syn immunoreactive substances were observed in both the nerve fibers and nerve plexus in the wall of the digestive tract of cynomolgus monkeys. The α-Syn immunopositive structures were found in the mucosa,submucosal nerve plexus,and myenteric nerve plexus of the digestive tract. The amounts of α-Syn in different regions of the digestive tract were different,with the higher level of α-Syn found in the duodenum,jejunum,ileum,and colon,and lower levels of stomach and esophagus.Conclusion α-Syn is mainly localized in the intrinsic nerves in the digestive tract of cynomolgus monkeys,with the levels differing in different regions of the digestive tract.

关 键 词：Α-突触核蛋白 消化道 食蟹猴 壁内神经 

分 类 号：R338[医药卫生—人体生理学]