HPLC法同时测定氯柳酊中氯霉素和水杨酸的含量  

Simultaneous determination of chloramphenicol and salicylic acid in tincture by HPLC

作　　者：马萍[1] 吴诚[1] 王宇[2] 王景成 曹淼[1] 孙平 牛华英 

机构地区：[1]第二炮兵总医院药学部,北京100088 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]济南大成医药发展有限公司,山东济南250100

出　　处：《中国药物应用与监测》2015年第5期271-274,共4页Chinese Journal of Drug Application and Monitoring

基　　金：军队医疗机构制剂标准提高科研专项课题计划重点项目(13ZJZ22)

摘　　要：目的：建立HPLC法同时测定氯柳酊中氯霉素和水杨酸的含量。方法：色谱柱为C18柱（4.6 mm×250 mm,5μm）;流动相为甲醇-水-冰醋酸（50∶50∶1）;流速为1.0 m L·min-1;柱温为30℃;检测波长为295 nm;进样量为10μL。结果：氯霉素和水杨酸分离良好。氯霉素在60.77-141.80μg·m L-1范围内线性关系良好（r=0.999 8）,水杨酸在123.50-288.10μg·m L-1范围内线性关系良好（r=0.999 7）,氯霉素和水杨酸的平均回收率分别为99.76%、99.87%,RSD分别为1.08%（n=9）、1.19%（n=9）。结论：本方法简便准确,快速可靠,可用于氯柳酊的含量测定和质量控制。Objective： To establish the HPLC method for simultaneous determination of chloramphenicol and salicylic acid in tincture. Methods： HPLC was used in the quantitative analysis with C18 chromatographic column（4.6 mm × 250 mm, 5 μm）. The mobile phase consisted of methanol-water-glacial acetic acid（50 ： 50 ： 1） with the flow rate of 1.0 m L·min-1. The column temperature was 30 ℃. The detection wavelength was 295 nm and the injection volume was 10 μL. Results： The calibration curves of chloramphenicol and salicylic acid had a good linearity in the range of 60.77 – 141.80 μg·m L-1（r = 0.999 8） and 123.50 – 288.10 μg·m L-1（r = 0.999 7） respectively. The average recovery of chloramphenicol was 99.76% with RSD of 1.08%（n = 9）. The average recovery of salicylic acid was 99.87% with RSD of 1.19%（n = 9）. Conclusion： The method is simple, accurate and reliable, which can be used as the quality control method of this preparation.

关 键 词：氯柳酊 氯霉素 水杨酸 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学] O657.72[理学—分析化学]