内皮祖细胞在噪声性耳聋耳蜗损伤中的变化  

Changes of endothelial progenitor cells in cochlea of noise deafness

作　　者：石峰[1] 李冬梅 杨锐睿[3] 苏玉静[4] 费秀静 

机构地区：[1]河北省唐山市协和医院耳鼻喉科,河北唐山063000 [2]河北省唐山市开滦职业病防治院耳鼻喉科,河北唐山063301 [3]河北省唐山市开滦总医院林西医院重症医学科,河北唐山063100 [4]河北省唐山市开滦总医院林西医院手术室,河北唐山063100 [5]河北省唐山市开滦总医院赵各庄医院外科,河北唐山063100

出　　处：《中国医药导报》2015年第31期30-34,共5页China Medical Herald

摘　　要：目的探讨内皮祖细胞在噪声性耳聋耳蜗损伤中的变化。方法 50只SD大鼠分为两组,其中空白对照组10只,实验组40只。空白对照组不做处理,实验组进行噪声暴露构建噪声性耳聋动物模型,并按照暴露时间分为4组,分别为暴露后即刻组和暴露后1 d组、暴露后7 d组、暴露后14 d组,每组各10只。分别检测各组动物的外周血内皮祖细胞水平和血管内皮生长因子、诱导型一氧化氮合酶及CD34水平,并进行比较。结果暴露后即刻组和暴露后1 d组、暴露后7 d组的外周血内皮祖细胞水平均显著高于空白对照组,差异有统计学意义(P<0.05);但暴露后14 d组与空白对照组比较,差异无统计学意义(P>0.05)。暴露后1 d组、暴露后7 d组的血管内皮生长因子表达水平均显著高于空白对照组,差异有统计学意义(P<0.05);但暴露后即刻组、暴露后14 d组与空白对照组比较,差异无统计学意义(P>0.05)。免疫组化结果显示:实验组噪声暴露后即刻、暴露后l d的CD34均呈微弱表达,噪声暴露后7 d呈强表达;噪声暴露后14 d呈阳性表达;噪声暴露后即刻的诱导型一氧化氮合酶呈微弱表达,暴露后1 d呈强表达,暴露后7、14 d组呈阳性表达。经扫描电镜结果显示:空白对照组动物的内耳内外毛细胞均呈现出整齐排列的情况,暴露后即刻组、暴露后1 d组可见内耳毛细胞呈现出紊乱状态,暴露后7 d组、暴露后14 d组,经观察动物耳蜗第二圈可见外毛细胞呈现出紊乱、缺失等现象。结论噪声性耳聋会导致耳蜗损伤的出现,内皮祖细胞数量会随着时间的变化发生改变,并在耳蜗损伤修复中发挥重要作用。Objective To explore the changes of endothelial progenitor cells in noise deafness cochlea injure. Methods50 SD rats were divided into two groups,the blank control group with 10 rats,and the experiment group with 40 rats.Blank control group received no intervention,experiment group exposed to noise and constructed noise deafness animal model,accoding to the exposed time,the rats were divided into four groups,after exposuring immediately group,after exposuring 1 d group,after exposuring 7 d group,after exposuring 14 d group,with 10 rats in each group. The peripheral blood endothelial progenitor cells,blood vascular endothelial growth factor,inducible nitric oxide synthase and CD34 of animals were detected and compared. Results The peripheral blood endothelial progenitor cells in after exposuring immediately group,after exposuring 1 d group and after exposuring 7 d group were higher than that in blank control group,the differences were statistically significant（P 〈0.05）; But there was no significant difference between after exposuring 14 d group and blank control group（P 〉0.05）. The vascular endothelial growth factor expression levels in after exposuring 1 d group,after exposuring 7 d group were higher than that in blank control group,the differences were statistically significant（P〈 0.05）; But those in after exposuring immediate group and after exposuring 11 d group were compared with those in blank control group,the differences were not statistically significant（P 〉0.05）. The result of immunohistochemistry showed： in experiment group,after exposuring immediately and 1 d,expression of CD34 was weak,after exposuring 7 d was strong,after exposuring 14 d was positive; Expression of inducible nitric oxide synthase after exposuring immediately was weak,after exposuring 1 d was strong,after exposuring 7,14 d was positive. The results of scanning electron microscopy showed： the inner ear and inner hair cells of animal in the blank control group showed a neatly arranged,inner ear hair cells in

关 键 词：噪声性耳聋 耳蜗损伤 内皮祖细胞 变化 

分 类 号：R245[医药卫生—针灸推拿学]