杨梅素对肝癌细胞Bel-7402裸鼠移植瘤的抑制作用  

Inhibition of Myricetin on tmnsplantation tumor of nude mice with human hepatoma cell

作　　者：李有富[1] 李魏林[1] 

机构地区：[1]湖北文理学院附属襄阳市中心医院药学部,湖北襄阳441021

出　　处：《中国医药导报》2015年第31期44-47,共4页China Medical Herald

摘　　要：目的 研究杨梅素（Myr）体内外对人肝癌Bel-7402细胞增殖、凋亡及机制的影响。方法 体外培养人肝癌Bel-7402细胞,MTT和SRB法检测细胞增殖抑制率;采用25只SPF级BALB/C-nu裸鼠建立Bel-7402细胞裸鼠异种移植瘤模型,分为5组,对照组,5-氟尿嘧啶（5-Fu,25 mg/kg）阳性对照组以及Myr（20、40、80 mg/kg）三个剂量组。腹腔注射给药3周后,处死裸鼠,剥瘤称重并计算抑瘤率。TUNEL法检测肿瘤组织细胞凋亡;FACS检测肿瘤组织细胞周期;免疫组织化学法检测Bax和Bcl-2含量。结果MTT和SRB法检测显示,Myr可以抑制Bel-7402细胞体外增殖并呈剂量依赖。腹腔注射给药3周后,Myr各剂量组瘤质量与模型组比较,差异有高度统计学意义（P〈0.01）;Myr体各剂量组Bel-7402细胞凋亡率与对照组比较,差异有高度统计学意义（P〈0.01）,Myr将细胞周期阻滞在G2-M期。Myr可以促进Bax蛋白表达（P〈0.05或P〈0.01）,抑制Bcl-2蛋白表达（P〈0.05或P〈0.01）。结论 杨梅素可抑制Bel-7402细胞的增殖,诱导其凋亡,其凋亡机制涉及Bcl-2家族。Objective To study the influence of Myricetin（Myr） on human hepatoma Bel-7402 cell multiplication,apoptosis and its mechanism in vivo and in vitro. Methods Bel-7402 cells were cultured in vitro,MTT and SRB were performed to observe the proliferation. The 25 BALB/C-nu nude mice of SPF lever were made Bel-7402 cell xenografts model,and they were divided into five groups,control group,5-Fu（25 mg/kg） positive control group,Myr（20,40,80 mg/kg）three doses groups. After 3 weeks drug abdominal injection,killed mice,the tumor was weighed and tumor-inhibition rate was counted. TUNEL was used for measuring the apoptosis,FACS was used for measuring the cell cycle; Immunohistochemistry was used for detecting Bax and Bcl-2 content. Results By MTT and SRB detection,Myr could inhibit Bel-7402 cell proliferation in vitro,and it showed dosage dependent. After 3 weeks drug abdominal injection,tumor mass of Myr（20,40,80 mg/kg） three doses groups were compared with control group,the differences were statistically significant（P 〈0.01）; apoptosis rate of Bel-7402 cell of Myr（20,40,80 mg/kg） three doses groups were compared with control group,the differences were statistically significant（P 〈0.01）,Myr arrested cell cycle in G2-M phase. Myr promoted Bax expression（P 〈0.05 or P 〈0.01）,and inhibited Bcl-2 expression（P 〈0.05 or P 〈0.01）. Conclusion Myr can inhibit the proliferation of Bel-7402 cells and induce apoptosis,the mechanism involves Bcl-2 family.

关 键 词：杨梅素 BEL-7402细胞 裸鼠 增殖 凋亡 BAX Bcl-2 

分 类 号：R285.5[医药卫生—中药学]