改良小鼠肾移植模型的建立  

Improvement on Kidney Transplantation Model in Mice

作　　者：黄健兵[1] 丁芳宝[1] 刘浩[1] 梅举[1] 

机构地区：[1]上海交通大学医学院附属新华医院心胸外科,上海200092

出　　处：《实验动物与比较医学》2015年第5期374-377,共4页Laboratory Animal and Comparative Medicine

基　　金：上海市科委基金（13XD1403200）

摘　　要：目的改进小鼠腹腔肾脏移植的手术方法，提高手术成功率。方法选用25对C57BL／6小鼠行同种同品系肾脏移植手术，8只C57BL／6小鼠切除肾脏作对照。简化供体。肾获取方法，从供体肾背侧开始分离，并减少肾动静脉游离范围，肾动脉开口以血管片方式获取。改进动静脉吻合技术，将供体’肾动、静脉分别与受体腹主动脉、下腔静脉行端侧吻合。尿道重建将供体肾输尿管末端引入膀胱内，固定输尿管壁于膀胱外膜。分两期切除受体自身’肾脏。结果手术成功率为92％，总手术时间为47．6±8．7min，热缺血时间3～5S，冷缺血时间31．1±6．3min。结论小鼠腹腔异位肾脏移植手术方法的改进能提高手术成功率，易于学习掌握，值得推广应用。Objective To improve the technique of kidney transplantation model in mice and to increase the success rate of operation. Methods Totally 25 pairs of C57BL/6 mice were used for kidney transplantations, and 8 C57BL/6 mice were used for control after nephrectomy. To simplify the donor kidney acquisition method, begin to separate the donor kidney from the dorsal side, reduce the separation range of the renal arteriovenous. Obtain the artery at a vascular patch. Improve the arteriovenous anastomosis technique. The donor renal artery and vein is sutured to the recipient abdominal aorta and inferior vena cava respectively by end to side anastomosis. The urethral ends of the donor were put into the recipient bladder to reconstruct the ureter, and fix the ureteral wall to the outer membrane of the bladder. The natural kidneys of the recipients were excised by two phases. Results The success rate of operation was 92%, the total operation time was 47.6±8.7 min, warm ischemia time was 3-5 s, cold ischemia time was 31.1±6.3 min. Conclusion The improvement technique of kidney transplantation model in mice can improve the success rate of operation. It is easy to learn and is worth to be widely applied.

关 键 词：小鼠 肾 移植 动物模型 

分 类 号：R699.2[医药卫生—泌尿科学] R-332[医药卫生—外科学]