亚高原习服大、小鼠消化管组织学观察  

Histological Observation on Digestive Tract of Sub-plateau Acclimatized SD Rats and KM Mice

作　　者：徐文漭[1] 王媛媛[1] 黎贵芸[1] 李涛[1] 蔡琳[1] 王力[1] 崔静[1] 李霞[2] 

机构地区：[1]成都军区昆明总医院病理科,云南省实验动物病理检测中心,昆明650032 [2]成都军区昆明总医院全军创伤骨科研究所,昆明650032

出　　处：《实验动物与比较医学》2015年第5期378-382,402,共6页Laboratory Animal and Comparative Medicine

基　　金：云南省心用基础研究自筹项目（2011FZ323）

摘　　要：目的探讨亚高原习服SD大鼠及KM小鼠消化管的组织学特征，为毒理学、新药安全性评价等提供依据。方法选取按照现行实验动物质量国家检测标准检测合格的SD大鼠20只、KM小鼠20只，按饲养地区分为平原组及亚高原组各10只，分别处死并剖检。选取消化管组织经体积分数10％甲醛固定，常规石蜡切片，HE染色，显微镜下观察组织结构和细胞的异同。结果（1）炎症是消化管普通存在的自发性病变，亚高原组炎症反应较平原组重。（2）亚高原组舌肌内链核增多，肌间隙增宽。（3）亚高原组小肠上皮黏膜内有杯状细胞化生。（4）亚高原组肌胃内更容易观察到肥大细胞浸润。其余组织学结构未见明显差异。结论SD大鼠与KM小鼠消化管组织学结构基本相同。亚高原组与平原组在消化管的组织学结构上存在一定的差异，主要表现为细胞和组织的适应性反应，研究人员在进行实验研究、药物安全性评价时应予充分考虑。Objective To explore histological characters of the digestive tract of Sub-plateau acclimatized SD rats and 20 KM mice and to provide comparative data for pathological inspection, toxicological studies and drug safety evaluation. Methods Twenty SD rats and 20 KM mice were divided into flatland group and sub-plateau group, each group include 10 ones. Animal quality and breeding condition complied with the state standard without any infectious disease after quarantine. Tissues of digestive tract were collected after euthanasia and autopsy. Tissues were fixed with 10% neutral formalin, the pathologic slide specimens were prepared by HE staining after paraffin imbedding, and then which were observed and studied under the light microscope. The same and different histological structure characters of them were summarized by comparative study. The histological structures of digestive tract were observed by light microscopy. Results （1） Inflammation was a common spontaneous lesion of digestive tract in all groups, but imflammation in the sub-plateau groups were more serious than the flatland groups. （2） Widening of space between lingualis, and more nuclear chains of lingualis were seen in sub-plateau groups. （3） Goblet cell metaplasia existed in intestinal mucous membrane of sub-plateau groups. （4） The mast cells were observed much easier in muscle stomach of sub-plateau groups. Conclusion The digestive tract characters of SD rat and KM mice are basically the same, but there are some histological differences in digestive tract between the flatland and sub-plateau group, which is an adaptive response of cell and tissue, and this difference should be fully considered in experimental research, toxicological studies and drug safety evaluation.

关 键 词：大鼠 小鼠 消化管 平原 亚高原 比较组织学 

分 类 号：Q95-33[生物学—动物学]