利用羟基磷灰石纳米管修复大鼠颅骨骨缺损实验观察  

Experimental Observation on Repair of Calvarial Defects by Hydroxyapatite Nanotube

作　　者：朱彦丞 郑欣[1] 陈一心[1] 王晓波[1] 邱旭升[1] 施鸿飞[1] 王骏飞[1] 桂雪洋 

机构地区：[1]南京医科大学鼓楼临床医学院,南京210008

出　　处：《实验动物与比较医学》2015年第5期390-393,共4页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金项目（81401793）;江苏省社会发展基金项目（BE2011604）;江苏省六大人才高峰项目（2012-WS-092）;南京市卫生局项目（YKK13079、ZKX12016）

摘　　要：目的观察羟基磷灰石纳米管（HANT）对大鼠颅骨缺损修复成骨的作用。方法采用自主合成的HANT（专利号：Z1201010123277．5），选用12只3月龄SD大鼠，在其双侧颅骨顶部对称部位各建立直径为5mm的缺损。采用自身对照的方法，在大鼠左侧颅骨缺损内植入0．5gHANT粉末为实验侧，右侧为空白对照侧，单纯制作颅骨缺损，不植入任何材料。在术后的12周进行CT的影像学观察分析，术后12周处死大鼠，取出颅骨，行组织病理学分析。结果术后大鼠均未发生感染死亡；术后各期植入区CT扫描实验侧颅骨缺损处均可见HABIT粉末，密度较骨组织低；病理学分析显示对照侧可见骨缺损处有纤维细胞填充，未见骨缺损愈合；实验侧颅骨缺损内侧壁有少量软骨细胞生长，骨缺损处可见未降解HANT粉末，大量纤维细胞长入包裹材料。结论HANT具有良好的生物相容性，但单纯应用HANT无法完全修复大鼠颅骨缺损，其成骨能力有待进一步实验改进及验证。Objective To investigate the ostegcnesis by hydroxyapatite nanotube（HANT） in a calvarial defect model. Methods Bilateral full-thickness defects （5 mm in diameter） of parietal bone were created in twelve three -month-old SD rats. The left defected side was filled with 0.5 g HANT （patent number： Z1201010123277.5） as experimental side and the right side was set as control. CT were taken at 12 weeks postoperation. The rats were euthanized at 12 weeks after surgery and the specimens were harvested for patholologic analysis. Results There was no infection or death on the surgical models. CT examination demonstrated that the residual HANT powder could be seen on the experimental side of the skull defect, but the density is lower than bone. Pathologic analysis showed that bone defect in the control side was filled with fiber cells. The powder partly degraded in the experimental side, few chondrocytes were found, and a large number of fiber cells enwrapped the powder. Conclusion The HANT has good biocompatibility, but singly implanting the nanotube can＇t completely repair the skull defect of rats. Therefore, its osteogenic ability need to be refined in further experiment.

关 键 词：羟基磷灰石纳米管(HANT) 大鼠 颅骨缺损 

分 类 号：R318.08[医药卫生—生物医学工程] R-332[医药卫生—基础医学]