2010～2013年上海地区实验大鼠小鼠病原体感染情况分析  

Analysis on Pathogen Infection of Mice and Rats in Shanghai from 2010 to 2013

作　　者：冯丽萍 陶凌云 冯洁 谢建云 林金杏 王胜昌 周洁 魏晓锋 高诚 邢正弘 

机构地区：[1]上海实验动物研究中心,上海201203

出　　处：《实验动物与比较医学》2015年第5期398-402,共5页Laboratory Animal and Comparative Medicine

基　　金：面向实验动物研究领域的大数据共享服务子平台应用及示范（13DZ1512104）;上海市实验动物资源共享服务平台（13DZ2291200）;国家科技支撑计划子课题：实验动物质量保证条件关键技术研究（2011BA115803）

摘　　要：目的了解上海地区实验大鼠小鼠病原体感染情况。方法对委托和抽样的上海地区清洁级和SPF级大鼠小鼠依据国家标准进行病毒、细菌和寄生虫检测。结果2010～2013年，上海地区实验大鼠小鼠主要检出的病原菌有金黄色葡萄球菌、绿脓杆菌、支原体和泰泽病原体；主要检出的病毒有小鼠肝炎病毒、仙台病毒等；主要检出的寄生虫有蠕虫、鞭毛虫、体外寄生虫。尽管小鼠的金黄色葡萄球菌阳性率呈现下降趋势，但是泰泽病原体和支原体的抗体阳性率都呈现增加趋势。大鼠泰泽病原体、仙台病毒抗体阳性率及大鼠蠕虫阳性率逐年增加。结论从病原体感染情况来看，上海地区实验大鼠小鼠质量有待提高，实验动物监管部门应进一步加强对上海地区实验大鼠小鼠的管理和质量监测。Objective To investigate the pathogen infection status of laboratory mice and rats in Shanghai. Methods The quality monitoring for viruses, bacteria and parasites in SPF or clean rats and mice was conducted according to national standards, in which the method, equipment and reagents were referring to the requirements of national standards. Results From 2010 to 2013 in Shanghai, the mainly identified pathogens infecting the mice and rats included the Staphylococcus aureus, Pseudomonas aeruginosa, Mycoplasma and Tyzzer＇s organism. And the mainly identified viruses infecting the mice and rats were mouse hepatitis virus and Sendai virus etc. Meanwhile, the mainly identified parasites were all Helminths, Flagellates and Ectoparasites. During this period, the detection rate of Staphylococcus aureus presented the downtrend in mice, but the detection rate of Tyzzer＇s organism and Mycoplasma showed uptrend. In addition, the detection rate of Tyzzer＇s organism, Sendai virus and all Helminths in rats increased year by year during this period. Conclusion In Shanghai region the quality of laboratory mice and rats needs to be improved. And the regulatory authorities in laboratory animal should strengthen the administration and quality monitoring of the rats and mice in the Shanghai.

关 键 词：实验动物 病原体检测 阳性率 

分 类 号：R95-33[医药卫生—药学]