NF-κB通路中TAK1靶点对胃癌细胞系AGS增殖的抑制作用  

Inhibitory Effect on the Proliferation of Gastric Carcinoma Cell Line AGS by Targeting TAK1 of NF-κB Signaling Pathway

作　　者：张晓娜[1] 张学彦[2] 于旸[3] 乔虹[1] 胥景峰 

机构地区：[1]哈尔滨医科大学第二附属医院内分泌科,黑龙江哈尔滨150086 [2]哈尔滨医科大学第二附属医院消化内科,黑龙江哈尔滨150086 [3]哈尔滨医科大学遗传学教研室,黑龙江哈尔滨150086

出　　处：《现代生物医学进展》2015年第28期5432-5434,5483,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨NF-κB信号传导通路中转化生长因子激活激酶1(TAK1)靶点对胃癌AGS细胞系增殖的影响。方法:利用si RNA沉默胃癌AGS细胞系中的TAK1基因,通过Western Blot验证细胞中TAK1基因的沉默情况,双项荧光素酶报告基因系统检测TAK1基因沉默对AGS细胞系NF-κB信号传导通路活性的影响,软琼脂克隆形成实验检测沉默TAK1基因对胃癌AGS细胞系成瘤能力的影响,MTT法检测沉默TAK1基因对胃癌AGS细胞系增殖能力的影响。结果:与对照组Sis-Con细胞相比,TAK1基因沉默组Sir-TAK1-1/Sir-TAK1-2细胞中NF-κB信号传导通路活性明显受抑制;TAK1基因沉默组Sir-TAK1-1/Sir-TAK1-2细胞软琼脂克隆形成的集落数目明显减少,差异具有统计学意义(P<0.05),且细胞生长的速度明显减慢。结论:TAK1是NF-κB信号传导通路激活的关键因子,沉默TAK1基因可以抑制AGS胃癌细胞系的成瘤性和增殖力。Objective： To investigate the effect of transforming growth factor-activated kinase 1（TAK1） of NF- κB signaling pathway on the proliferation of AGS gastric cancer cell. Methods： TAK1 gene of AGS cells was silenced by si RNA and then confirmed by western blot. The effect of silencing TAK1 gene on the activity of NF-κB signaling pathway, cell tumorigenicity and cell proliferation were determined by dual-luciferase reporter gene system, soft agar clone formation assay and MTT assay, respectively. Results： The activity of NF- κB signaling pathway in Sir-TAK1-1/Sir-TAK1-2 cells was significantly inhibited after silencing TAK1 gene when compared with Sis-Con group cells. Moreover, the number of clone formation of Sir-TAK1-1/Sir-TAK1-2 cells was dramatically decreased, and significant differences were found compared with Sis-Con group cells（P〈0.05）. The growth of Sir-TAK1-1/Sir-TAK1-2cells was significantly decreased. Conclusion： TAK1 gene is the key factor for activating NF-κB signaling pathway. The tumorigenicity and proliferation of AGS gastric cancer cell lines could be inhibited by silencing TAK1 gene.

关 键 词：转化生长因子激酶1(TAK1) NF-ΚB 胃癌 

分 类 号：R-33[医药卫生] R735.2