心舒宝对大鼠心肌梗死的保护作用及其药理机制  

The Protective Effect and Pharmacological Mechanism of Xinshubao on Myocardial Infarction of Rats

作　　者：李艳梅[1,2,3,4] 陈志亮 赵明一[6] 彭忠禄[7] 陈志衡[6] 刘琳[6] 蔡骞[6] 齐绪峰[1,2,3,4] 

机构地区：[1]暨南大学再生医学教育部重点实验室,广东广州510632 [2]科技部及广东省科技厅"国际科技合作基地",广东广州510632 [3]再生医学联合实验室(暨南大学-香港中文大学),广东广州510632 [4]暨南大学发育与再生生物学系,广东广州510632 [5]漳州片仔癀药业股份有限公司,福建漳州363000 [6]中南大学湘雅三医院,湖南长沙410006 [7]湘南学院文学化生系,湖南郴州423000

出　　处：《激光生物学报》2015年第5期448-454,共7页Acta Laser Biology Sinica

基　　金：福建省科技重点项目(基金编号:2011Y5003)

摘　　要：目的:探讨心舒宝对大鼠心肌梗死的保护作用及其药理机制,为临床用药提供理论依据。方法:0.25g/m L心舒宝灌胃二周后诱导大鼠心肌梗死模型,二周后进行大鼠心肌组织的Masson’s Trichrome染色实验;利用胰酶消化方法分离大鼠心脏原代内皮细胞,并培养于培养液。用0.005%,0.01%,0.05%的心舒宝处理细胞后,缺氧培养24 h、48 h,流式细胞仪分析其凋亡率。结果:心舒宝处理组的大鼠心肌梗死面积及其切面胶原明显小于对照组(P<0.01);0.005%心舒宝处理的大鼠心脏原代内皮细胞在缺氧48 h后,其凋亡明显降低(P<0.01)。结论:心舒宝可能通过降低大鼠心脏原代内皮细胞的凋亡而发挥保护大鼠心肌梗死的作用。Objective: To investigate the the protective effect and Pharmacological mechanism of Xinshubao on myocardial infarction of rats,and provide a theoretical basis for clinical use. Methods: The model of myocardial infarction rats were induced by 0. 25 g / m L Xinshubao after two weeks,then cardiac muscle Masson's Trichrome staining was done using trypsin digest and to isolate the rat heart primary endothelial cells,and cultured the cells in 1 640 medium. Flow cytometry analysis the apoptosis rate after Hypoxia culture 24 h,48 h of cells treated with 0. 005%,0. 01%,0. 05%Xinshubao for 24,28 h. Results: Xinshubao treated rat myocardial infarction area and the section of collagen was significantly less than the control group( P < 0. 01); 0. 005% of Xinshubao treated rat heart primary endothelial cells in hypoxic for 48 h,apoptosis Octreased significantly( P < 0. 01). Conclusion: Xinshubao may protect myocardial infarction rats by inhibiting apoptosis of rat heart primary endothelial cells.

关 键 词：心舒宝 心肌梗死 细胞凋亡 

分 类 号：R972[医药卫生—药品]