金鱼Dishevelled3基因的克隆与分化表达研究  

A Study on Cloning and Differential Expression of Gene Dishevelled 3 in Goldfish

作　　者：邓美霞[1] 张卉琴 张翠媛[1] 李林芳[1] 钟彬彬[1] 陈思[1] 周柳[1] 肖亚梅[1] 李万程[1] 刘文彬[1] 

机构地区：[1]湖南师范大学生命科学学院蛋白质化学与鱼类发育生物学重点实验室,湖南长沙410081

出　　处：《激光生物学报》2015年第5期460-468,共9页Acta Laser Biology Sinica

基　　金：湖南省科技计划资助项目(2014FJ3084);湖南省教育厅资助科研项目(13C523)

摘　　要：目的:本文主要以金鱼中的dvl-3基因为研究对象,研究dvl-3在金鱼不同的组织和胚胎发育时期中可能行使的功能。方法:采用RACE克隆技术获得金鱼dvl-3基因的c DNA全长,再利用半定量RT-PCR技术和蛋白免疫印记(Western blot)技术分析研究dvl-3基因在mRNA水平和蛋白水平上的表达差异。结果:在金鱼不同组织与胚胎发育时期,dvl-3基因在mRNA水平和蛋白水平上具有显著的表达差异性。结论:由此可见散乱蛋白dvl-3在金鱼不同的组织和胚胎发育时期中可能行使不同的功能,但其具体的功能及机制还有待进一步研究。Objective: Here,our study focused on gene dvl-3 in Carassius auratus. Research the function of dvl-3 proteins during embryonic development stages in Carassius auratus as well as in different tissues. Method: Firstly,we used RACE( rapid-amplification of c DNA ends) clone technology to obtain full-length c DNA of gene dvl-3,and then semiquantitative RT-PCR and western blot were used to analyze the differential expression of dvl-3 in mRNA level and protein level respectively. Results: The results showed that: Semi-quantitative RT-PCR and semi-quantitative western blot results are different in different embryonic development and different tissues. Conclusions: Thus it showed that the function of dvl-3 proteins varied during embryonic development stages in Carassius auratus as well as in different tissues.However,the exact mechanism remained to be further explored.

关 键 词：散乱蛋白 dvl-3 WNT信号转导通路 RT-PCR WESTERNBLOT 

分 类 号：Q78[生物学—分子生物学]