自身免疫性疾病患者免疫学指标和血清细胞因子的检测及临床意义  

Clinical significance of immunological indexes and serum cytokines in patients with autoimmune diseases

作　　者：宋爱华[1] 王昕升[1] 李艳静[1] 

机构地区：[1]河北省秦皇岛市第三医院,066000

出　　处：《河北医药》2015年第22期3379-3381,共3页Hebei Medical Journal

摘　　要：目的检测自身免疫性疾病患者外周血CD+4CD+25调节性T细胞、T淋巴细胞亚群、免疫球蛋白、补体、炎性因子及黏附分子的变化,探讨上述指标在疾病发生发展中的作用。方法选取2012年1月至2015年1月收治的150例自身免疫性疾病患者,根据疾病活动情况分为活动期组80例,稳定期组70例,另选取同期进行健康体检的正常人150例为对照组。采用流式细胞术测定3组外周血CD+4CD+25调节性T细胞、T淋巴细胞亚群(CD+3、CD+4、CD+8)及NK细胞水平。采用免疫透射比浊法测定免疫球蛋白(Ig A、Ig G、Ig M)及补体(C3、C4)水平。采用酶联免疫吸附试验测定炎性因子[肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)、白介素-17(IL-17)、γ干扰素(IFN-γ)]及黏附分子[细胞黏附分子-1(ICAM-1)、血管内皮细胞黏附分子-1(VCAM-1)、E-选择素(E-selectin)]水平。结果活动期组与稳定期组、对照组比较,CD+4CD+25调节性T细胞、CD+4、CD+4/CD+8、NK细胞、C3、C4明显降低,CD+8、Ig A、Ig G、Ig M、TNF-α、IL-10、IL-17、IFN-γ、ICAM-1、VCAM-1、E-selectin明显升高,差异有统计学意义(P<0.05)。结论自身免疫性疾病患者存在炎性反应及免疫功能紊乱,对免疫相关指标及炎性因子、黏附分子的检测有助于评估疾病活动期患者的免疫状态,为判断疾病进展及临床治疗提供实验依据。Objective To observe the changes of CD4＋ CD2＋ 5 FoxP3 ＋ regulatory T cells （Treg）, T lymphocyte cells subsets, immunoglobulin, complements,＇inflammatory cytokines, complements and adhesion molecules in patients with autoimmune diseases in order to explore their roles in pathogenesis and development of diseases. Methods A total of 150 patients with autoimmune diseases who were admitted into our hospital from January 2012 to January 2015 were enrolled in the study. These patients were divided into active period group （ n = 80） and stable period group （ n = 70 ） according to the active status of diseases, besides, 150 healthy subjects were served as control group. The CD4＋ CD2＋5 FoxP3 ＋ Treg, T lymphocyte cells subsets （CD3＋ , CD4＋ , CD8 ） and NK cells were detected by flow cytometry. Immunoglobulins （IgA, IgG, IgM） and complements （ C3, C4） were measured by turbidimetric method with automatic biochemical analyzer. Inflammatory cytokines （TNF-oL, IL-10, IL-17, IFN-3,） and adhesion molecules （ICAM-1,VCAM-1,E-selectin） were detected by ELISA. Results As compared with those in control group and stable period group, CD4＋ CD2＋ 5 FoxP3 ＋ Treg, CD4＋ , CD4/CD8, NK cells, C3 and C4 in active period group were siguificanfly decreased,however, CD8＋ , IgA, IgG, IgM, TNF-α, IL-10, IL-17, IFN-γ, ICAM-1, VCAM-1 and E-selectin in active period group were significantly increased （ P 〈 0.05 ）. Conclusion There exists inflammatory reaction and immunological function disorder in patients with autoimmune diseases, therefor the detection of immune indexes, inflammatory cytokines and adhesion molecules is helpfor to assess the patient＇ s immune state of and disease＇ s progress so as to provide experimental basis for clinical treatment.

关 键 词：自身免疫性疾病 免疫功能 细胞因子 临床意义 

分 类 号：R593[医药卫生—内科学]