HPLC梯度洗脱法同时测定丹芎跌打膏中4种主要成分的含量  

Simultaneous Determination of Four Main Ingredients in Danxiong Dieda Gao by HPLC

作　　者：马灵珍 

机构地区：[1]亳州职业技术学院药学院,安徽亳州236800

出　　处：《安徽科技学院学报》2015年第4期33-37,共5页Journal of Anhui Science and Technology University

基　　金：安徽省高等学校教学质量与教学改革工程实训中心建设项目(20101460)

摘　　要：目的：建立了同时测定丹芎跌打膏中4个主要成分（京尼平龙胆双糖苷、栀子苷、去甲氧基姜黄素和姜黄素）含量的高效液相色谱检测方法。方法：采用依利特C18柱,流动相为乙腈（A）和0.4%冰醋酸溶液（B）,梯度洗脱,流速为1.2 mL/min;柱温为30℃;检测波长λ1=238 nm（京尼平龙胆双糖苷和栀子苷）,λ2=430 nm（去甲氧基姜黄素和姜黄素）。结果：在优化的色谱条件下,京尼平龙胆双糖苷、栀子苷、去甲氧基姜黄素和姜黄素的线性范围分别为4.10-82.00μg/mL（r=0.9993）、5.69-113.80μg/mL（r=0.9999）、7.80-156.00μg/mL（r=0.9995）和8.06-161.20μg/mL（r=0.9998）;4种成分的平均加样回收率（n=6）分别为97.89%、99.24%、97.16%、96.89%,RSD分别为1.16%、0.97%、1.56%、1.23%。结论：该方法为丹芎跌打膏的质量控制提供了科学依据。Objective： To establish a HPLC method for the simultaneous determination of four main components（ Genipin- 1- β- D- gentiobioside,geniposide,demethoxycurcumin and curcumin） in Danxiong Dieda Gao.Method： The HPLC separation was performed on a Hypersil C18 column eluted with the mobile phases of acetonitrile（ A）- 0. 4% acetic acid solution（ B） in a gradient elution and the flow rate was 1. 2 mL·min- 1. The column temperature was at 30 ℃. Genipin- 1- β- D- gentiobioside and geniposide were detected at 238 nm,demethoxycurcumin and curcumin were detected at 430 nm. Results： The chromatographic peaks of four main components and the corresponding spectra were ideal under the optimal conditions. Genipin- 1- β- D- gentiobioside,geniposide,demethoxycurcumin and curcumin had good linearity at 4. 10 - 82. 00 μg / mL（ r = 0. 999 3）,5.69 - 113. 80 μg / mL（ r = 0. 999 9）,7. 80 - 156. 00 μg / mL（ r = 0. 999 5）,8. 06 - 161. 20 μg / mL（ r = 0. 9998）,respectively. The average recoveries and RSD were 97. 89%（ 1. 16%）,99. 24%（ 0. 97%）,97. 16%（ 1.56%） and 96. 89%（ 1. 23%）,respectively. Conclusion： The method can provide reference for evaluating the quality of Danxiong Dieda Gao.

关 键 词：丹芎跌打膏 京尼平龙胆双糖苷 栀子苷 去甲氧基姜黄素 姜黄素 

分 类 号：R284.1[医药卫生—中药学]