反应时间对聚多巴胺/纳米银修饰多孔钛生物学性能的影响  

The Influen ce of Reaction Time on the Biocompatibility of Polydopamine/Silver Nanoparticles Modified Porous Titanium

作　　者：容锡沧[1] 张余[2] 谭帼馨[3] 谭英[3] 宁成云[4] 李梅[2] 李丽华[2] 

机构地区：[1]南方医科大学附属江门市人民医院,广东省江门市529000 [2]广州军区广州总医院骨科医院,广东省广州市510010 [3]广东工业大学化工轻工学院,广东省广州市510006 [4]华南理工大学材料科学与工程学院,广东省广州市510641

出　　处：《组织工程与重建外科杂志》2015年第5期295-300,319,326,共8页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家自然科学基金项目(81271957)

摘　　要：目的探讨制备新型钛基材料过程中,纳米银颗粒载入反应时间对其体外抗菌性能和细胞相容性等生物学性能的影响。方法通过聚合作用以多巴胺在纳米孔钛表面构建聚多巴胺(PDA)膜层,通过调节纳米银颗粒载入反应时间(15 min,30 min,60 min),得到不同纳米银颗粒浓度的聚多巴胺修饰钛。利用扫描电镜(SEM)、细胞黏附、细胞毒性及杀菌率等检测方法,对载不同纳米银颗粒浓度的聚多巴胺修饰钛进行体外生物相容性及抗菌性能评价。结果载入纳米银反应时间为30 min的钛基材料较其他反应时间的材料表现出良好的细胞黏附能力、较低的细胞毒性和满意的抗菌性能。结论通过调整纳米银沉积反应时间,可调节材料表面银离子释放量,可得到既有良好细胞相容性,又有较强抗菌能力的聚多巴胺/纳米银修饰多孔钛。Objective To investigate the reaction time influence on the antibacterial property and cytocompatibility of polydopamine/silver nanoparticles modified porous titanium. Methods A bioinspired polydopamine （PDA） layer was deposited on titanium surface by polymerization. By adjusting the response time of nanosize silver particles （15 min, 30 min, 60 min） of the modified titanium, different concentration of silver nanoparticles modified titanium coated by polydopamine were prepared. Using scanning electron microscopy （SEM）, cell adhesion, cell toxicity and sterilization rate examination to evaluate the in vitro biocompatibility and antibacterial properties of polydopamine modified titanium with load of different concentration of silver nanoparticles. Results The modified titanium surface material with the response time of 15 min in nanosize silver particles loading, showed better cell adhesion ability, lower cytotoxicity and more satisfactory antibacterial properties than the others. Conclusion By adjusting the reaction time of nano silver deposition, the silver ion release quantity is adjustable, and the polydopamine/silver nanoparticles modified porous titanium, which have good cell compatibility and strong antibacterial ability, can be gained.

关 键 词：多孔钛 聚多巴胺 纳米银颗粒 生物学性能 反应时间 

分 类 号：R318.08[医药卫生—生物医学工程]