长白山复序橐吾多糖的提取及其免疫活性研究  

Study on the Extraction and Immunological Activity of Polysaccharides from Ligularia jaluensis in Changbai Mountains

作　　者：朱梅[1] 任桂红[1] 赵桂云[1] 孙海明[1] 董然[2] 刘洪章[2] 

机构地区：[1]北华大学化学与生物学院,吉林省吉林市132013 [2]吉林农业大学生命科学学院,吉林长春130118

出　　处：《长春师范大学学报》2015年第10期66-70,共5页Journal of Changchun Normal University

基　　金：吉林省科技发展计划项目(20100254);吉林省教育厅科学技术研究项目(2012138)

摘　　要：经90%乙醇浸泡处理的复序橐吾叶,热水煮提,乙醇沉淀,常规干燥,得粗多糖,由理化性质分析表明,粗多糖为不含淀粉的酸性杂多糖;粗多糖经冻融法和酶-Sevag法联合脱蛋白及透析处理后,通过DEAE-纤维素离子交换层析、Sepharose CL-6B凝胶柱层析分级纯化,得到6个多糖级分;采用高效液相色谱法测定各级分的单糖,组成为甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖、木糖、岩藻糖;进一步利用高效凝胶渗透色谱法对各多糖级分的分子量分布进行了分析;应用MTT法对复序橐吾多糖免疫活性进行测定,结果表明其对小鼠脾脏T淋巴细胞的体外增殖有一定的促进作用。Ligularia jaluensis leaves were soaking treated by 90% ethanol,then Crude polysaccharide were obtained by hot aqueous extraction,ethanol precipitation and conventional desiccation. Phisicochemical property analysis indicated that Ligularia jaluensis crude polysaccharide was acidic heteropolysaccharide without starch. 6 polysaccharide fractions were obtained via fractionation purification of deae- cellulose and Sepharose CL- 6B Gel chromatography after deproteinzation through combination of freeze- thaw method and enzyme- Sevag method and dialysis. Monosaccharide composition of each polysaccharide fraction was determined by HPLC to consist of mannose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose,arabinose,xylose and fucose. MWD of each polysaccharide fraction were analysed further by HPGPC. The result of determination of immunological competence manifested that Ligularia jaluensis polysaccharide showed some auxo- action to mice T- cell proliferation in vitro.

关 键 词：复序橐吾 多糖 分离提取 理化性质 免疫活性 

分 类 号：Q539[生物学—生物化学]