吸入布地奈德对肥胖哮喘小鼠气道炎症及瘦素水平的影响  

Effect of inhaled budesonide on the airway inflammation and the expression of leptin of obese asthma mice

作　　者：苏毅[1] 杜雪梅[1] 郝月琴[1] 唐华平[1] 

机构地区：[1]青岛市市立医院呼吸科,山东青岛266071

出　　处：《中华实用诊断与治疗杂志》2015年第11期1091-1094,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：山东省自然科学基金(ZR2014HM105)

摘　　要：目的 探讨吸入布地奈德对肥胖哮喘小鼠气道炎症和气道重建的影响,以及瘦素与肥胖哮喘小鼠激素抵抗的关系。方法 75只清洁级3-4周龄雌性C57/6J小鼠随机分为正常体质量的对照组、肥胖组、哮喘组、肥胖哮喘组和布地奈德干预肥胖哮喘组（干预组）,每组15只。对照组采用普通饲料喂养,生理盐水致敏激发;肥胖组采用高脂饮食诱导建立肥胖模型,生理盐水致敏激发;哮喘组采用普通饲料喂养,卵白蛋白致敏激发法建立哮喘模型;肥胖哮喘组、干预组在高脂饮食诱导同时给予卵蛋白致敏激发建立肥胖哮喘模型,其中干预组每次雾化前1h雾化吸入0.5g/L布地奈德混悬液30min。各组小鼠于末次激发48h内取肺泡灌洗液,计数白细胞计数、嗜酸性粒细胞和中性粒细胞比率,采用ELISA法检测血清瘦素水平,组织病理检查观察小鼠气道炎性浸润情况。结果肥胖哮喘组肺泡灌洗液中白细胞计数[（22.99±3.93）×104/mL]、嗜酸性粒细胞比率[（19.63±3.71）%]、中性粒细胞比率[（8.93±2.46）%]、血清瘦素水平[（2 114.20±189.15）ng/L]、气道管壁厚度[（12.80±1.12）μm2/μm]、气道平滑肌厚度[（5.58±1.37）μm2/μm]均明显高于对照组[（6.58±1.03）×104/mL、（2.93±2.03）%、（4.90±2.00）%、（1 438.54±208.14）ng/L、（5.02±0.78）μm2/μm、（1.92±0.88）μm2/μm]、肥胖组[（7.66±0.88）×104/mL、（3.03±1.78）%、（7.10±2.79）%、（1 948.93±246.51）ng/L、（5.21±0.82）μm2/μm、（2.24±0.73）μm2/μm）]和哮喘组[（19.68±2.91）×104/mL、（14.87±4.13）%、（5.83±2.02）%、（1 677.75±246.51）ng/L、（11.24±0.79）μm2/μm、（4.76±1.18）μm2/μm],差异均有统计学意义（P〈0.05）,血清瘦素水平与气道管壁厚度、气道平滑肌厚度呈正相关（r=0.625,P=0.015;r=0.767,P=0.002）;干预组小鼠肺泡灌洗液嗜酸性粒细胞比率[（15.57±3.76）%]明显低于肥胖哮喘组,中性粒细�Objective To explore the effect of inhaled budesonide on the airway inflammati and airway remodeling as well as to analyze the relationship between leptin and glucocorticoid resistance in obese mice with asthma. Methods Seventy- five female C57/6J mice aged 3 to 4 weeks were randomly divided into control group with normal body mass, obese group, asthma group, obese asthma group and intervention group, with 15 mice in each group. Intervention group with obese asthma mice was intervened by budesonide. Control group was fed with normal diet and was sensitized and challenged with normal saline. Obese group was fed with high-fat diet and was sensitized and challenged with normal saline. Asthma group was fed with normal diet and was sensitized and challenged with ovalbumin （OVA）. Obese asthma group and intervention group were fed with high-fat diet and were sensitized and challenged with OVA to establish obese asthma models. Intervention group received aerosolize budesonide 0. 5 g/L for 30 minutes one hour before OVA challenge. All mice were sacrificed 48 hours after the last challenge to obtain bronchoalveolar lavage fluid （BALF） for calculating total white blood cell count, eosinophil counts and neutrophil counts. The serum leptin level was determined by ELISA method. The pathological inflammatory infiltration change of airway was observed by histopathologic method. Results The total white blood cell count （（22. 99± 3. 93） ×10^4/mL）, eosinophil count （（19. 63 ± 3. 71）%） and neutrophil count （（8.93±2.46）%） in BALF, the serum leptin level （（2 114.20±189.15） ng/L）, total area of tracheal wall/basement membrane perimeter （WAt/Pbm） （（12.80±1.12） μm^2/μm）, and airway smooth muscle area/ basement membrane perimeter （WAm/Pbm） （（5.58±1.37） μm^2/μm） in obese asthma group were significantly higher than those in control group （ （6.58 ±1.03） × 10^4/mL, （2.93±2.03） %, （4.90±2.00） %, （1 438. 54 ± 208.14） ng/L, （ 5.02 ± 0.78）μm^2/

关 键 词：肥胖 哮喘 瘦素 布地奈德 小鼠 

分 类 号：R562.25[医药卫生—呼吸系统] R589.2[医药卫生—内科学]