大鼠肾移植后外周血T细胞亚群动态变化  

Changes of T lymphocyte subsets in peripheral blood after renal transplantation in rats

作　　者：张艾莎 刘新华[1] 陈继红[1] 顾凤娟 隋晓露 胡磊[1] 努尔孜叶.阿布里克木 热依汉.西里甫 

机构地区：[1]新疆医科大学第一附属医院肾病科,乌鲁木齐830054

出　　处：《中华实用诊断与治疗杂志》2015年第11期1095-1097,1099,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：乌鲁木齐市科学技术计划基金(Y131310009)

摘　　要：目的探讨肾移植术后大鼠T细胞亚群动态变化与急性排斥反应的关系。方法 15只健康SD大鼠为正常对照组;15只行假手术SD大鼠为假手术组;16只SD大鼠作为供者、16只SD大鼠作为受者建立同系基因移植为非急性排斥组;16只SD大鼠为受体建立异系基因移植为急性排斥组,供体为16只Wistar大鼠。监测4组大鼠术后第7天T细胞亚群及肾功能变化,并对其组织病理学急性排斥反应严重程度进行半定量评分。结果急性排斥组在术后第7天血清肌酐[(115.35±30.09)μmol/L]、尿素氮[(19.80±10.71)mmol/L]水平和急性排斥反应半定量评分[3(3,3)分]均明显高于正常对照组[(17.80±4.45)μmol/L、(4.97±0.49)mmol/L、0(0,0)分]、假手术组[(47.06±4.28)μmol/L、(5.75±1.06)mmol/L、0(0,0)分]和非急性排斥组[(55.31±8.48)μmol/L、(8.25±1.57)mmol/L、0(0,1)分](P均<0.01);急性排斥组CD4+T细胞比率[(27.98±6.07)%]和CD4+/CD8+比值(2.13±0.66)明显高于非急性排斥组[(8.23±2.63)%、0.20±0.06]、假手术组[(9.84±2.87)%、0.22±0.10]和正常对照组[(8.94±2.44)%、0.20±0.07],CD8+T细胞比率[(13.85±3.74)%]明显低于非急性排斥组[(43.08±6.26)%]、假手术组[(46.67±6.94)%]和正常对照组[(43.69±9.82)%](P均<0.01)。结论 T细胞亚群检测对评价肾移植术后免疫功能状态有一定价值,有助于肾移植术后急性排斥反应的早期诊断和治疗。Objective To analyze the relationship between the changes of T lymphocyte subsets and acute rejection （AR） after renal transplantation in rats. Methods Control group consisted of 15 normal SD male rats. Sham operation group consisted of 15 SD rats. Non-AR group consisted of 16 SD rats receiving SD syngenegic renal grafts. AR group consisted of 16 SD rats receiving allogenegic renal grafts from 16 Wistar rats. T lymphocyte subsets and kidney function were detected. The degree of acute histopathologic rejection was semiquantitatively scored. Results The levels of serum creatinine （（115.35±30.09）μmol/L）, urea nitrogen （（19.80±10.71） mmol/L） and AR semiquantitative score （3 （3, 3） ） were significantly higher by day 7 after operation in AR group than those in control group （（17.80±4.45） μmol/L, （4.97±0.49） mmol/L, 0 （0, 0））, sham-operation group （（47.06±4.28） μmol/L, （5.75±1.06） mmol/L, 0 （0, 0）） and non-AR group （（55.31±8.48）μmol/L, （8.25±1.57） mmol/L, 0 （0, 1）） （P〈0.01）. The proportion of CD4^＋ in T lymphocytes and the CD4^＋/CD8^＋ ratio were significantly higher in AR group （（27.98±6.07） %, 2.13±0.66） than those in non-AR group （（8.23±2. 63）%, 0.20±0.06）, sham-operation group （（9. 84±2. 87）%, 0. 22±0. 10） and control group （（8.94±2.44）%, 0. 20±0. 07） （P〈0.01）. The proportion of CD8^＋ in T lymphocytes was significantly lower inARgroup （（13.85±3.74）%） by day 7 day after operation than that in non-AR group （（43.08±6.26）%）, sham-operation group （（46.67±6.94）%） and control group （（43.69±9.82）^） （P〈0.01）. Conclusion The detection of T lymphoyte subsets has a significant value to evaluating the immunity of recipients undergoing renal transplantation,contributing to the early diagnosis and treatment of AR.

关 键 词：肾脏移植 急性排斥反应 大鼠 T细胞亚群 

分 类 号：R699.2[医药卫生—泌尿科学]