Roux—en—Y胃转流术对代谢综合征大鼠糖脂代谢及过氧化物酶体增长因子活化受体γ表达的影响  

Effects of Roux-en-Y gastric bypass surgery on the glycolipid metabolism and expression of peroxisome proliferator-activatived receptor-γ protein in rats with metabolic syndrome

作　　者：张立海[1] 夏宾[1] 王娇[1] 黄文丽[1] 王跃生[1] 孙国娟[1] 谭柏宏 尹彦斌[1] 

机构地区：[1]佳木斯大学附属第一医院普通外科一科佳木斯大学糖尿病外科治疗研究所,154003

出　　处：《中华消化外科杂志》2015年第11期935-940,共6页Chinese Journal of Digestive Surgery

基　　金：黑龙江省自然科学基金面上项目（D201127）;佳木斯大学科学技术校级面上项目（1321201540）;佳木斯大学研究生创新基金（LM2015034,LM2015030）

摘　　要：目的探讨Roux-ell-Y胃转流术对代谢综合征大鼠肥胖相关指标、糖脂代谢指标、器官脂肪组织过氧化物酶体增长因子活化受体γ（PPAR-en-γ）蛋白表达的影响。方法40只健康雄性7。8周龄sD大鼠，适应性喂养1周后，选取30只，予高糖高脂高盐饲料配合20％蔗糖水喂养12周，筛选出24只符合标准的代谢综合征模型大鼠，按完全随机区段分组法分为假手术组（11只）和手术组（13只）。假手术组行胃窦十二指肠离断原位吻合术，手术组行Roux-en-Y胃转流术。其余10只健康SD大鼠予标准饮食喂养设为正常组。术后5周处死大鼠，立即取肠系膜、双肾周及双侧附睾旁脂肪组织，采用Westernblot法检测大鼠器官脂肪组织PPAR-γ蛋白含量。观察指标包括：（1）肥胖相关指标：术前及术后5周进食量、体质量、腹围、Lee＇s指数，术后5周器官脂肪质量、器官脂肪系数。（2）糖脂代谢指标：术前及术后5周空腹血糖、血清胰岛素、稳态胰岛素评价指数（HOMA-IR）、TC、TG、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、血清游离脂肪酸。（3）术后5周器官脂肪组织PPAR-1蛋白含量。正态分布的计量资料以面±s表示，多组间比较采用方差分析，两组间比较采用LSD．t检验，重复测量数据采用重复测量方差分析。结果手术组大鼠术后1周死亡3只，死亡率为3／13；其余各组无大鼠死亡。正常组大鼠进食量、体质量、腹围、Lee＇s指数术前分别为（26．9±1．5）g、（499±46）g、（18．2±0．4）cm、309±9，术后5周分别为（27．7±2．1）g、（547±43）g、（18．6±0．5）em、312±8；假手术组大鼠上述指标术前分别为（26．1±1．8）g、（584±42）g、（19．3±0．6）em、（317±13），术后5周分别为（28．8±1．7）g、（677±39）g、（20．6±0．7）cm、334±11；手术组大鼠上述指标术前分别为（25．9±2．1）g、（579±47Objective To investigate the effects of Roux-en-Y gastric bypass surgery on the obesity-related indexes, glycolipid metabolism indexes and expression of peroxisome proliferator-activatived receptor-γ （PPAR-γ） protein in visceral adipose tissues of rats with metabolic syndrome. Methods After 40 Sprague Dawley （SD） male rats with the age of 7-8 weeks were adaptively fed for 1 week, 30 rats were selected randomly and were fed with high-fat, high glucose and high salt diet combined with sucrose water for 12 weeks, and then 24 rats with met- abolic syndrome were screened for eligibility and were allocated into the model group （ n = 11 ） and the operation group （n =13） based on the randomized complete block design. Rats of the model group and operation group received transection and reanastomosis of the gastrointestinal tract and Roux-en-γ gastric bypass surgery, respectively. Other 10 rats receiving normal feeding were divided into the control group. After executing rats at postoperative week 5, the levels of PPAR-γ protein in visceral adipose tissues around mesentery, double kidneys and bilateral epididymis were detected by the Western blot. Observation indexes： （1） obesity-related indexes included food intake before operation and at postoperative week 5, body weight, abdominal circumference, Lee＇s index, visceral adipose weight at postoperative week 5 and visceral adipose coefficient. （2） Glycolipid metabolism indexes included fasting plasma glucose before operation and at postoperative week 5, fasting serum insulin, homeostasis model assessment-insulin resistance （ HOMA-IR）, total cholesterol （ TC）, triglyceride （ TG）, high density lipoprotein- cholesterol （HDL-C）, low density lipoprotein- cholesterol （LDL-C） and free fatty acid （FFA）. （3） Level of PPAR--/protein in visceral adipose tissues at postoperative week 5. Measurement data with normal distribution were presented as x± s. Comparison among groups was analyzed using the ANOVA, and comparison between

关 键 词：代谢综合征 胃转流术 器官脂肪 糖脂代谢 过氧化物酶体增长因子活化受体γ 

分 类 号：R656.61[医药卫生—外科学]