欧美山杨杂种插穗不定根2个发育时期基因表达谱分析  

Gene expression profile of aspen hybrid cuttings in two developing stages of the adventitious root

作　　者：闫绍鹏[1] 尚艳茹 冷淑娇[1] 杨瑞华[2] 王秋玉[1] 

机构地区：[1]东北林业大学生命科学学院 [2]黑龙江省农业科学院园艺分院

出　　处：《北京林业大学学报》2015年第10期9-13,共5页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(J1210053);中央高校基本科研业务费专项(2572014CA22)

摘　　要：以欧美杂种山杨嫩枝插穗为实验材料,进行扦插不定根生成过程中插穗形态和解剖学观察,通过Solexa高通量测序对扦插后6和12 d插穗不定根生成部位进行转录水平上的检测分析。插穗解剖观察发现扦插后6 d为根原基的诱导期,12 d为不定根的伸长生长期,对插穗不定根2个时期高通量测序获得差异表达基因2 328个。对筛选出的显著差异表达基因进行功能注释、分类和KEGG及pathway分析,确定2个山杨样本中差异表达基因参与的生化代谢和信号转导途径。We used Populus tremula × P. tremuloides softwood cuttings as experimental material, and observed morphological and anatomical changes of the cuttings during the rooting process of adventitious roots. Solexa high-throughput sequencing was performed for the parts of cuttings where adventitious roots generated 6 days and 12 days after cutting（ DAC） at transcriptional level. Anatomical observation showed that the induction stage of the adventitious root primordia was 6 days after cutting and the elongation stage of adventitious roots was 12 days after cutting. A total of 2 328 differentially expressed genes were obtained by the Solexa high-throughput sequencing. The significantly differential expression genes were classified after their functions annotation,and then the KEGG passway analysis was carried out. On this basis,the biochemical metabolic and signal transduction pathways of these differentially expressed genes were determined.

关 键 词：杂种山杨 基因表达谱 扦插 差异表达 

分 类 号：S718.43[农业科学—林学]