脑源性神经营养因子基因单核苷酸多态性与陕西汉族海洛因依赖群体的相关性研究  

Association of single nucleotide polymorphisms of brain-derived neurotrophic factor gene with heroin dependence in the Han population of Shaanxi Province

作　　者：李云肖 殷芳圆 乔晓孟 魏曙光[1] 赖江华[1] 

机构地区：[1]西安交通大学医学部法医学院,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2015年第6期787-791,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.NSFC 81172910)~~

摘　　要：目的探讨脑源性神经营养因子（BDNF）基因多态性与海洛因依赖的相关性。方法对符合纳入及排除标准的海洛因依赖组308例及健康对照组317例进行临床资料收集与血样采集。采用飞行时间质谱分析方法对BDNF rs16917204、rs6265、rs7103873、rs7103873、rs56164415、rs13306221、rs2030323共7个多态性位点进行分型,数据采用SPSS 20.0和HaploView 4.0软件进行统计学分析。结果 rs6265位点基因型频率分布在海洛因依赖组及健康对照组中存在统计学差异（χ2=15.151,P=0.001）。与健康对照组相比,海洛因依赖组rs6265位点G等位基因频率显著高于健康对照组（χ2=9.864,P=0.002,OR=1.429,95%CI=1.143~1.786）。rs13306221位点基因型频率分布在海洛因依赖组及健康对照组中存在统计学差异（χ2=7.699,P=0.006）。与健康对照组相比,海洛因依赖组G等位基因频率显著高于健康对照组（χ2=7.137,P=0.008,OR=0.539,95%CI=0.340~0.853）。连锁不平衡检验发现,由rs6265与rs71038731（block1）构建的单倍型为强连锁不平衡（D〉0.9;r2〉0.8）。在海洛因依赖组及健康对照组中,G-G单倍型频率分布存在显著性差异,健康对照组中G-G单倍型频率显著高于海洛因依赖组（χ2=4.546,P=0.033）。结论 BDNF基因rs6265及rs13306221多态性位点可能与海洛因依赖有关,携带有rs6265位点G等位基因及rs13306221位点G等位基因的个体更易形成海洛因依赖,携带有G-G单倍型（rs6265-rs71038731）的个体相对不易对海洛因产生依赖。Objective To examine the association between the polymorphisms of brain-derived neurotrophic factor（BDNF）gene with heroin dependence.Methods Genomic DNA was isolated from the venous blood leukocytes of 308 unrelated patients with heroin dependence and 317 healthy individuals.Seven single nucleotide polymorphisms（SNPs）were genotyped using MassARRAY system.Data were analyzed using HaploView4.0and SPSS 20.0software.Results There was a significant difference in the genotype frequency of rs6265 between heroin dependence group and healthy control group（χ2=15.151,P=0.001）.The rs6265 G allele was significantly higher than in controls（χ2=9.864,P=0.002,OR=1.429,95% CI=1.143-1.786）.Furthermore,there was also a significant difference in the genotype frequency of rs13306221 between heroin dependence group and control group（χ2=7.699,P=0.006）.The rs13306221 G allele was significantly higher than in controls（χ2=7.137,P=0.008,OR=0.539,95% CI=0.340-0.853）.Strong linkage disequilibrium（LD）was observed in one block（D＇ 〉0.9;r〉20.8）,and significantly less G-G haplotype frequency of block1（χ2=4.546;P=0.033）was found in heroin dependence group.Conclusion Our findings support the role of BDNF rs6265 and rs13306221 polymorphisms in heroin dependence and may guide future studies to identify other genetic risk factors for heroin dependence.

关 键 词：脑源性神经营养因子(BDNF) 海洛因依赖 单核苷酸多态性 

分 类 号：R89[医药卫生—法医学]