4种植物激素对紫苏叶愈伤组织诱导及黄酮积累的影响  

Effect of Four Plant Hormone on Callus Induction and Flavonoid Formation in Perilla Frutescens Leaves

作　　者：郭云贵[1] 程晓敏[1] 王泽琼[1] 于娅[1] 韩晓红[1] 

机构地区：[1]武汉生物工程学院生物科学与技术学院,湖北武汉430415

出　　处：《江汉大学学报（自然科学版）》2015年第5期460-464,共5页Journal of Jianghan University：Natural Science Edition

基　　金：武汉市市属高校产学研项目(CXY201434)

摘　　要：以紫苏叶为外植体,采用单因素和正交设计试验研究了植物激素对紫苏叶愈伤组织诱导和黄酮积累的影响。结果表明:4种激素对紫苏叶愈伤组织诱导的影响依次为2,4-D>6-BA>NAA>KT;对愈伤组织积累黄酮的影响依次为2.4-D>6-BA>KT>NAA;紫苏叶愈伤组织培养的最佳激素组合为6-BA 2.0 mg/L+2,4-D 0.5 mg/L+KT 0.6 mg/L+NAA 0.8 mg/L;经愈伤组织培养获得的黄酮含量最高达到3.925%,远高于自然界紫苏叶黄酮含量。上述试验结果说明利用组培技术获得紫苏黄酮有可行性。In this study, with the leaves of Perilla frutescens were used as explant, effect of different concentrations of the hormone on inducing callus and formating flavonoid in Perilla frustescens leaves were studied by single factor and orthogonal test. It was found that the effect order of four kind of hormones to callus induction was 2,4-D〉6-BA〉NAA〉KT and the order to flavonoid formation of callus was 2,4-D 〉 6-BA 〉 KT 〉 NAA, the optimum medium for callus induction was 6-BA 2. 0 mg/L ＋ 2,4- D 0. 5 mg/L＋KT 0. 6 mg/L＋NAA0. 8 mg/L by orthogonal design, under the optimum conditions, the highest indution rate was 83.3 % after 25 days. Furthemore, the flavonoid formation of callus achieved at 3. 925 %, it is much higher than the natural Perilla frustescens leaves, the experimental results indicate the feasibility of flavonoid formation with tissue engineering.

关 键 词：紫苏叶 植物激素 黄酮 愈伤组织 正交试验 

分 类 号：Q813.12[生物学—生物工程]