HPLC法测定通络降脂胶囊中总黄酮醇苷的含量  

Determination of total flavonoid glycoside in tongluojiangzhi capsules by HPLC

作　　者：倪玉佳 吴晓燕[1] 金婷[1] 瞿发林[1] 

机构地区：[1]解放军第102医院,江苏常州213003

出　　处：《实用药物与临床》2015年第10期1228-1231,共4页Practical Pharmacy and Clinical Remedies

基　　金：军队医疗机构制剂标准提高科研专项课题(13ZJZ16-3)

摘　　要：目的 建立以高效液相色谱法测定通络降脂胶囊中总黄酮醇苷含量的方法. 方法 色谱柱为Lichrospher C18（150 mm ×4. 6 mm,5 μm）,流动相为甲醇-0. 4%磷酸-乙腈（39：56：5）,流速为1. 0 mL/min,检测波长为360 nm,进样量20 μL,柱温为30 ℃. 结果 槲皮素、山柰素、异鼠李素分别在4. 548 × 102 -4. 548 × 103 ng、3. 46 × 102 -3. 46 × 103 ng、65. 6-656 ng 进样量范围内呈良好的线性关系（ r =0. 999 9、r =0. 999 8、r =0. 999 2,n=5）,平均加样回收率分别为 101. 0% （RSD =1. 90%）、97. 2% （RSD =1. 66%）、97. 8% （RSD =1. 86%）. 结论 所建立的方法简便、准确、重复性好,可用于通络降脂胶囊中总黄酮醇苷的含量测定.Objective To establish an HPLC method for the determination of total flavonoid glycoside in tongluojiangzhi capsules. Methods The method was used with the chromatographic conditions of a Lichrospher C18 column and methanol-0. 4% phosphoric acid-acetonitrile （39：56：5） as the mobile phase,the flow rate was 1. 0 mL/min,the column temperature was 30 ℃ and the detection wavelength was 360 nm. Results The linear ranges of quer-cetin,kaempferol and isorhamnetin were 4. 548 × 102 -4. 548 × 103 ng （r=0. 999 9,n=5）,3. 46 × 102 -3. 46 × 103 ng （r=0. 999 8,n=5）,65. 6-656 ng （r=0. 999 2,n=5）,respectively. The average recoveries of quercetin,kaempferol and isorhamnetin were respectively 101. 0%,97. 2%,97. 8%. Conclusion The method is simple,accurate and specif-ic,which can be used for the determination of total flavonoid glycoside in tongluojiangzhi capsules.

关 键 词：通络降脂胶囊 高效液相色谱法 槲皮素 山柰素 异鼠李素 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]