子宫腺肌病小鼠血管生成及雌激素效应因子的相互作用机制  

Changes of Angiogenesis and Estrogen Effect Factors and Their Relationship in Adenomyosis Mice

作　　者：付先芸[1,2] 魏绍斌[2] 

机构地区：[1]三峡大学,湖北宜昌443000 [2]成都中医药大学,四川成都610075

出　　处：《武汉大学学报（医学版）》2015年第6期883-886,917,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81273796)

摘　　要：目的:以雌激素效应相关因子、血管生成相关因子及其相互作用为靶点,探讨在子宫腺肌病(AM)小鼠疾病发生发展的不同时间段及子宫在位及异位内膜不同空间部位、疾病的主导机制。方法:以健康雌性未孕8周龄ICR小鼠为研究对象,行子宫内移植脑垂体手术,以建立AM的小鼠模型。随机设正常组、A组(3月模型组)、B组(6月模型组),采用免疫组化染色方法,检测各组AM小鼠异位和在位内膜雌激素效应相关因子、血管生成相关因子的表达变化。结果:与正常组相比,A组、B组在位内膜CD31均显著下调,VEGF、MMP-2显著上调,A组P450、ERα、COX-2无显著变化,B组P450、COX-2显著上调;异位内膜A组P450、ERα值显著增高。A组与B组比较,在位内膜CD31、VEGF、MMP-2值无显著差异,B组P450、ERα、COX-2均显著高于A组;异位内膜CD31、VEGF的表达A组与B组间比较无显著差异,MMP-2、COX-2值B组显著高于A组。无论是A组或B组,VEGF、MMP-2在位内膜增高幅度均显著高于同期异位内膜,P450表达A、B组异位内膜显著高于同期模型组在位内膜。结论:雌激素效应因子异常、血管生成相关因子异常及其相互作用,促成了AM的发生和发展,但在疾病的不同阶段,其主导机制有所不同。在位内膜的缺血缺氧可能为疾病的始动机制,导致异位内膜雌激素效应相关因子显著上调。Objective：To assay the levels of estrogen and angiogenesis related factors in adenomyosis mice,and to investigate their relationship and the role in the development adenomysis.Methods：Adenomyosis mouse model was established by transplanting isologous anterior pituitary glands（PGs）into the right uterine lumen on 8-week-age old ICR mouse.The angiogenesis indices（CD31,MMP-2,and VEGF）and the estrogen effect factors（P450,COX-2,and ERα）were detected by the immunohistochemisty method.Results：1As compared with normal control group,in the eutopic endometrium,the expression of CD31 decreased,VEGF and MMP-2increased significantly in adenomyosis mice;there was no significant change of P450,ERα,and COX-2after3 months of modeling,but after 6months,there was a significant up-regulation in P450 and COX-2.2 In the eutopic endometrium,there was no significant difference in CD31,VEGF,and MMP-2levels between 3-month and 6-month groups,while the P450,ERα,and COX-2levels was significant higher after 6months.In the ectopic endometrium,CD31 and VEGF had no significant difference between 3-month and 6-month groups,but MMP-2and COX-2levels were increased after six months.3At the same period of adenomyosis,the changes of VEGF and MMP-2were more significant in eutopic endometrium than in ectopic site,while the changes of P450 in ectopic site were more significant.Conclusion：Changes of indicators for angiogenesis and estrogen pathway were found in different time and different site of endometrium,which suggest that they may play a role in the pathogenesis of adenomyosis.

关 键 词：子宫腺肌病 小鼠模型 血管生成相关因子 雌激素效应相关因子 

分 类 号：R711.71[医药卫生—妇产科学]