右归丸对四氯化碳诱导的小鼠肝纤维化损伤的防护作用  

Protection effect of You-gui pill extract on carbon tetrachloride-induced liver damage mice

作　　者：叶聤杰 闫晓风[1] 胡旭东[1] 王晓玲[1] 

机构地区：[1]上海中医药大学基础医学院生物教研室,上海201203

出　　处：《中西医结合肝病杂志》2015年第5期289-290,300,I0001,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家"重大新药创制"专项项目(No.2009ZX09311-003);上海市教委预算内项目(No.2012JW01);上海中医药大学基础医学院"攀登计划"

摘　　要：目的:观察右归丸对四氯化碳(CCl4)诱导小鼠肝纤维化模型的作用。方法:C57雄性小鼠随机分为正常对照组,模型对照组,右归丸干预组(低剂量,中剂量,高剂量),共5组,CCl4复制肝损伤模型,于造模之日起开始右归丸干预组小鼠给予右归丸灌胃干预。造模结束后,摘眼球取静脉血清检测丙氨酸转氨酶(ALT),取其肝组织做天狼星红胶原染色以及羟脯氨酸(Hyp)含量测定。结果:与CCl4模型组比较,右归丸干预组小鼠肝组织胶原面积减小(<0.05);肝组织Hyp含量下降,血清ALT显著降低(<0.05),但没有剂量依赖差别。结论:右归丸对于CCl4小鼠肝纤维化有显著的防护作用。Objective：To observe the effect of You-gui pill extract on carbon tetrachloride（CCl4）induced liver fibrosis damage mice.Methods：Male C57BL/6 mice were randomly divided into normal group,model group,treatment groups;CCl4 was used to induce liver damage on mice,and treatment groups were given the You-gui pill extract by gastric intervention.Sirius red staining of collagen,hydroxyproline（Hyp）content determination were to observe the liver tissue pathological changes,and serum alanine transaminase（ALT）test was to examine liver function.Results：Compared with model group,collagen area,Hyp content of liver tissues and scram ALT in treatment group decreased significantly（ 〈0.05）.Conclusion：You-gui pill can significantly protect mice from liver fibrosis damage induced by CCl4.

关 键 词：右归丸/药效学 四氯化碳 肝纤维化 防护作用 小鼠 

分 类 号：R285.5[医药卫生—中药学]