菊花rbcL基因电子克隆及生物信息学、适应性进化分析  

In Silico Cloning,Bioinformatics and Adaptive Evolution Analysis of rbcL Gene in Chrysanthemum morifolium

作　　者：丁帅[1] 熊勇[1] 李正涛[1] 王忠诚[1] 孟亚媛 李乐[2] 肖焱波[1] 

机构地区：[1]云南民族大学化学与生物技术学院,昆明650500 [2]中北大学化工与环境学院,山西太原030051

出　　处：《种子》2015年第10期24-30,共7页Seed

基　　金：研究生创新基金项目(编号:2014YJY 77)

摘　　要：目的:利用电子克隆技术获得菊花叶绿体中编码核酮糖-1,5-二磷酸羧化氧化酶大亚基rbcL基因,并对该电子克隆的菊花rbcL基因序列、编码氨基酸的特性及适应性进化进行分析。方法:根据相关文献,利用电子克隆方法克隆出菊花rbcL完整基因序列,对其进行生物信息学分析、结构建模和评价。结果:通过电子克隆获得菊花rbcL基因完整的开放阅读框序列,该系列长1 452bp,编码氨基酸483个。在预测电子克隆菊花rbcL基因编码蛋白的二级结构中,主要有α螺旋19个,β折叠18个。通过适应性进化分析找到7个氨基酸正选择位点(228S,255V,279T,309M,328S,439T,449T)。结论:电子克隆获得菊花rbcL基因的完整序列为以后实验研究提供一定的参考数据,对所编码的蛋白质适应性研究有利于探究对菊科植物适应环境的机制。Objective：Ribulose-1,5-bisphosphate carboxylase/oxygenase（Rubisco）from Chrysanthemum morifolium was cloned in silico cloning.rbcLgene sequences in silico cloning,properties of encoding amino acid and adaptive evolution were analyzed.Method：According to the related literature,a full-length rbcLgene sequences form Chrysanthemum morifolium was cloned in silico cloning,and the structure and function of rbcLprotein were predicted by bioinformatics analysis.In the site-specific model,we found amino acid positive selection sites inrbcLgene.Result：A complete open reading frame sequences of electronic cloning chrysanthemumrbcLgene was 1 452 bp and encoded 483 amino acids.rbcLprotein secondary structure has 19α-helices and 18β-sheets.Seven amino acid sites（228S,255 V,279T,309 M,328S,439 T,449T）were found under positive selection.Conlusion：The complete sequence fromChrysanthemum morifolium rbcLgene in silico cloning provided certain reference data for further study,and encoded protein adaptability research was helpful to explore the mechanism of compositae adapting to the environment.

关 键 词：菊花 RBCL基因 电子克隆 生物信息学 适用性进化分析 

分 类 号：S682.11[农业科学—观赏园艺]