miR-171基因家族进化分析及靶基因预测  

Molecular Evolution of miR-171 Gene Family and Prediction of Their Target Genes

作　　者：王艳芳[1] 周瑞莲[1] 赵彦宏[2] 

机构地区：[1]鲁东大学生命科学学院,中国山东烟台264025 [2]鲁东大学农学院,中国山东烟台264025

出　　处：《生命科学研究》2015年第6期479-483,共5页Life Science Research

基　　金：国家自然科学基金资助项目(31201199);山东省自然科学基金项目(ZR2013CL013);水稻生物学国家重点实验室开放项目(120201)

摘　　要：运用生物信息学方法在miRBase中搜索植物miR-171基因家族的序列,分析miR-171序列的进化特征并预测其靶基因。结果表明,在38种植物中共搜索到219条miR-171序列,大部分miR-171基因都存在于基因间隔区。进化分析表明,miR-171基因家族的进化与物种进化关联不大。采用3个miRNA靶基因预测软件对大豆和玉米miR-171基因的靶基因进行预测,发现了miR-171可能参与生长因子、转录因子、蛋白酶等的调控,作用范围非常广泛。Sequences of miR-171 gene family were downloaded in miRBase by bioinformatics method. Molecular evolution of miR-171 gene family was analyzed and their target genes were predicted. The results showed that 219 sequences of miR-171 genes were found in 38 plant species totally. Most of the miR-171 genes Were located in the intergenie region （IGR）. There was no closely relationship found between molecular evolution of miR-171 gene family and the evolution of plants. Target genes of miR-171 gene family were predicted by three target gene prediction softwares in soybean and corn. The results found that miR-171 genes were involved in the regulation of growth factors, transcription factors, proteinase and other respects.

关 键 词：miR-171 基因家族 分子进化 靶基因预测 

分 类 号：Q752[生物学—分子生物学]