GFP荧光标记GLP-1受体细胞系的建立  

Establishment of a GFP Fluorescence Labeled GLP-1 Receptor Cell Line

作　　者：李明瑞[1] 季秀玲[1] 张敏[1] 张宽仁[1] 刘东波[2] 魏云林[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,中国云南昆明650504 [2]湖南农业大学国家中医药管理局亚健康干预技术实验室,中国湖南长沙410128

出　　处：《生命科学研究》2015年第6期501-504,共4页Life Science Research

摘　　要：为建立胰高血糖素样肽1受体(glucagon-like peptide 1 receptor,GLP-1R)药物筛选模型,真核表达载体pCMV6/GFP/GLP-1R构建好后,转染至U2OS细胞,转染后的细胞经G418筛选获得单克隆细胞株。该细胞株经Western-blot和GLP-1类似物检测,结果表明GLP-1R在该细胞株高表达并对GLP-1类似物有着高灵敏度与特异性的反应,可以用于GLP-1受体激动剂的筛选。To establish glucagon-like peptide-1 receptor （GLP-1R） cellular model for screening GLP-1R ligands. A eukaryotic expression vector pCMV6-GLP-1R-GFP was constructed and transfected into U2OS cells. A stable expressing GLP-1R-GFP U2OS cell line was established and the expression of GLP-1 receptor was confirmed by Western-blot with a GLP-1 specific antibody. The activities of expressed GLP-1RGFP were investigated with the cellular redistribution of green fluorescence after stimulation with GLP-1 analogs. The sensitivity and specificity of this cell line was qualified and the results showed that the cell line was suitable for GLP-1 receptor ligand screening.

关 键 词：胰高血糖素样肽-1受体(GLP-1R) GLP-1-GFP/U2OS细胞株 Ⅱ型糖尿病 

分 类 号：Q819[生物学—生物工程]