产碱性蛋白酶菌株的筛选与诱变育种  

Selection and Mutation Breeding of Alkaline Protease-producing Strains

作　　者：郑津辉[1] 

机构地区：[1]天津师范大学生命科学学院,天津300387

出　　处：《华北农学报》2015年第5期122-124,共3页Acta Agriculturae Boreali-Sinica

摘　　要：为获得性状稳定、高产的碱性蛋白酶菌株,采用平板诱变法对产碱性蛋白酶菌株进行诱变处理。结果表明,采用牛奶平板法及Folin测酶活法从鸡粪土样获得产碱性蛋白酶出发菌株。该菌株经亚硝基胍(NTG)诱变并利用利福平抗性株筛选获得蛋白酶活力为出发菌株34,65倍的2个变异株。因此,利用亚硝基胍作为诱变剂可产生高产酶菌株。In order to obtain stable high yield of alkaline protease strains,producing alkaline protease strain was treated by tablet mutagenesis method for mutagenic treatment. The results showed that by using milk tablet and Folin measuring enzyme activities,alkaline protease strains were produced from chicken dung upon sample. The strain by nitrosoguanidine（ the NTG mutagenesis） and use of rifampicin resistant strains screened two variants,whose protease activity was 34 times and 65 times of the starting strain. Consequently,mutagen nitrosoguanidine could produce high yield of alkaline protease strains.

关 键 词：碱性蛋白酶 筛选 诱变育种 

分 类 号：Q939.97[生物学—微生物学]