马甲子的化学成分研究  

Chemical constituents from Paliurus ramosissimus

作　　者：武蕊娟 宋联强 谭镭[2] 詹雁[2] 阮佳[2] 徐超群[2] 

机构地区：[1]成都中医药大学,四川成都610075 [2]四川省中医药科学院,四川成都610041 [3]北京以岭药业有限公司,北京102600

出　　处：《中草药》2015年第19期2834-2838,共5页Chinese Traditional and Herbal Drugs

基　　金：四川省科技支撑计划(15ZC1978);四川省科研院所科技成果转化资金项目(14010139);四川省公益性科研院所基本科研业务专项

摘　　要：目的研究马甲子Paliurus ramosissimus的化学成分。方法马甲子乙醇提取物通过反复硅胶柱色谱和高效液相色谱等方法进行分离纯化,根据理化性质及NMR、MS等波谱技术对化合物进行结构鉴定。并采用MTT法对新化合物2进行体外抗肿瘤活性实验。结果从马甲子乙醇提取物中分离得到2个化合物,分别鉴定为白桦脂酸(1)和2α,27-二反式对羟基肉桂酰氧基白桦脂酸(2)。化合物2对HepG-2细胞和He La细胞的IC50分别为20.34、14.83μmol/L。结论化合物2为新化合物,命名为马甲子素,属于白桦脂酸型五环三萜类化合物,化合物1和2均为首次从本植物中发现,化合物2表现出显著的体外抗肿瘤活性。Objective To study the chemical constituents in the ethanol extract from Paliurus ramosissimus. Methods The compounds were isolated and purified by repeated silica gel column chromatographies and HPLC. The structures of the compounds were identified on the basis of chemical and spectral methods of NMR, MS, etc. Combined with the MTT method, the inhibitory effect of compound 2 in vitro against the Hep G-2 and He La cells was assayed. Results Two new compounds were isolated from P. ramosissimus and identified as betulinic acid（1） and 2α,27-di（E-p-hydroxy cinnamoyloxy）-betulinic acid（2） for the first time. The IC50 values of compound 2 are 20.34 and 14.83 μmol/L against the Hep G-2 and He La cells. Conclusion Compound 2 is a new compound named as paliurusene, which belongs to triterpenoids and shows the obvious cytotoxicity in vitro against the cancer cell lines.

关 键 词：马甲子 白桦脂酸 马甲子素 五环三萜 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]