逍遥散中当归的抗抑郁活性成分的研究  

Antidepression constituents from Angelica Sinensis Radix in Xiaoyao Powder

作　　者：宫文霞[1,2] 周玉枝[1] 李肖[1,2] 高丽[1] 王月华[3] 田俊生[1] 高晓霞[1] 秦雪梅[1] 杜冠华[3] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006 [3]中国医学科学院药物研究所,北京100050

出　　处：《中草药》2015年第19期2856-2862,共7页Chinese Traditional and Herbal Drugs

基　　金：国家国际科技合作项目(2011DFA32630);"重大新药创制"科技重大专项(2012ZX09103201-035);国家自然科学基金资助项目(30901960);山西省科技攻关项目(20140313008-14);山西省科技基础条件平台建设项目(2014091022)

摘　　要：目的指认逍遥散中君药当归对复方抗抑郁有效部位的贡献成分,并筛选其中潜在的抗抑郁活性成分。方法采用UPLC-PDA技术对逍遥散抗抑郁有效部位及当归相应部位的指纹图谱进行匹配,通过对比色谱峰的保留时间及全波长紫外光谱图,归属当归对逍遥散抗抑郁有效部位的贡献成分。运用多种色谱方法对贡献成分进行导向分离,采用现代谱学技术鉴定化合物的结构,并对分离得到的化合物进行体外活性验证。结果共归属并鉴定出当归对逍遥散抗抑郁有效部位的贡献成分5个,分别为阿魏酸松柏醇酯(1)、E-丁烯基苯酞(2)、藁本内酯(3)、Z-丁烯基苯酞(4)、12-异戊烯酰基-14-乙酰基-2E,8E,10E-三烯-4,6-二炔-1-醇(5),其中化合物5为首次从伞形科植物中分离得到。化合物1、3、4能有效保护谷氨酸损伤的SH-SY5Y和PC12细胞。结论 UPLC-PDA导向分离技术可快速、准确指认复方来源于其单味药材的贡献成分。阿魏酸松柏酯、藁本内酯、Z-丁烯基苯酞可能为当归贡献逍遥散抗抑郁作用的活性成分。Objective This current study focused on the identification of active constituents from Angelica Sinensis Radix in Xiaoyao Powder based on UPLC-PDA-guided isolation technique. Methods The UPLC-PDA chromatogram of Xiaoyao Powder was compared with that of Angelica Sinensis Radix. The relative retention time of each peak and the Uhraviolet spectra provided by PDA were used in the analyses. The constituents were isolated from Angelica Sinensis Radix under the guidance of UPLC-PDA investigation. The structures of the isolates were elucidated by NMR techniques. The antidepression effect was evaluated on glutamate-induced neurons. Results Five marker peaks of Xiaoyao Powder fingerprint belonged to Angelica Sinensis Radix and they were determined as coniferyl ferulate（1）, E-butylidenephthalide（2）, ligustilide（3）, Z-butylidenephthalide（4）, and 14-acetoxy-12-senecioyloxytetradeca-2E,8E,10E-trien-4,6-diyn-1-ol（5）. Compound 5 was isolated from the plants in Umbelliferae for the first time. The treatment with compounds 1, 3, and 4 could protect PC12 and SH-SY5 Y cells from glutamate-induced cytotoxicity. Antidepression bioactivity of compound 1 was first investigated. Conclusion UPLC-PDA-guided isolation technique is confirmed to be a rapid and accurate method to identify the main active constituents from Angelica Sinensis Radix in Xiaoyao Powder.

关 键 词：逍遥散 当归 导向分离 抗抑郁 UPLC-PDA 阿魏酸松柏醇酯 E-丁烯基苯酞 藁本内酯 Z-丁烯基苯酞 12-异戊烯酰基-14-乙酰基-2E 8E 10E-三烯-4 6-二炔-1-醇 

分 类 号：R284.1[医药卫生—中药学]