傣药竹叶兰中1个新苯丙素及其抗烟草花叶病毒活性  

A new phenylpropanoid from Dai Medicine Arundina graminifolia and its anti-tobaco mosaic virus activities

作　　者：董伟[1] 周堃[1] 王月德 吉炳琨 周敏[1] 高雪梅[1] 胡秋芬[1] 叶艳青[1] 

机构地区：[1]云南民族大学民族药资源化学国家民委教育部重点实验室,云南昆明650500

出　　处：《中草药》2015年第20期2996-2998,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(21362044;21032085);云南省高校科技创新团队资助项目(IRTSTYN 2014-11)

摘　　要：目的对傣药竹叶兰Arundina graminifolia全株的化学成分进行研究,以期发现新的活性化合物。方法运用硅胶、凝胶、MCI-GEL树脂及PR-HPLC等多种色谱技术进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构,并采用半叶法检测化合物的抗烟草花叶病毒活性。结果从竹叶兰氯仿-甲醇(1∶1)提取物中分离得到4个苯丙素类化合物,分别鉴定为6-(3-羟基丙酰)-5-甲氧基-异苯并呋喃-1(3H)-酮(1)、香豆酸(2)、ω-羟基化愈创木丙酮(3)、3-甲氧基-4-羟基-苯丙醇(4)。结论化合物1为新化合物,命名为竹叶兰苯丙素,化合物1表现出明显的抗烟草花叶病毒活性,其相对抑制率为22.6%。Objective For the purpose of finding new bioactive agents from ethnic medicines, the chemical study on Dai Medicine Arundina graminifolia was carried out. Methods The chemical constituents in the whole plant of A. graminifolia were isolated by silica gel, MCI-GEL resin, Sephadex LH-20 column chromatography, and high performance liquid chromatography（HPLC） method. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. The inhibitory activities of compound 1 against tobaco mosaic virus（TMV） replication were tested using the half-leaf method. Results Four phenylpropanoids, 6-（3-hydroxypropanoyl）-5-hydroxymethyl-isobenzofuran-1（3H）-one（1）, coumaric acid（2）, ω-hydroxypropioguaiacone（3）, and 3-methoxy-4-hydroxy-propyl alcohol（4） were isolated from the whole plant of A. graminifolia. Conclusion Compound 1 is isolated from the whole plant of A. graminifolia for the first time. Compound 1 is a new compound named Arundina phenylpropanoid. It is tested for its anti-TMV activities. The results show that compound 1 exhibits the potential anti-TMV activities with the inhibitory rate of 22.6%.

关 键 词：竹叶兰 苯丙素 竹叶兰苯丙素 对羟基苯丙烯酸 抗病毒活性 烟草花叶病毒 

分 类 号：R284.1[医药卫生—中药学]