乙型肝炎患者血清乙型肝炎病毒-DNA与常用血清标志物的相关性分析  

Correlation between HBV-DNA and routine serum markers in patients with hepatitis B virus infection

作　　者：钟政荣[1,2] 徐云侠[3] 丁淑琴[2] 张伦军[2] 丁晓琳[2] 胡建国[2] 

机构地区：[1]上海交通大学医学院附属第三人民医院检验科,201999 [2]蚌埠医学院第一附属医院检验科,安徽蚌埠233004 [3]蚌埠医学院第一附属医院神经内科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2015年第10期1387-1389,共3页Journal of Bengbu Medical College

基　　金：安徽省自然科学研究项目(1308085MH124);安徽省教育厅自然科学研究重点项目(KJ2011A205)

摘　　要：目的:评估乙型肝炎(乙肝)患者血清乙肝病毒DNA(HBV-DNA)载量与血清免疫和生化标志物之间的关系。方法:收集214例乙肝患者血清,分别用荧光定量PCR法、化学发光法和速率法检测血清中的HBV-DNA、乙肝病毒表面抗原(HBs Ag)、乙肝病毒e抗原(HBe Ag)和丙氨酸氨基转移酶(ALT),分析各标志物之间的关系。结果:214例中,HBV-DNA阳性率为57.48%;HBe Ag阳性率为28.04%。HBV-DNA载量与HBs Ag和ALT水平均无相关关系(P>0.05),但与HBe Ag水平密切相关(P<0.01),HBV-DNA阳性组的HBe Ag和ALT水平均明显高于HBV-DNA阴性组(P<0.01)。结论:HBV-DNA载量与HBe Ag水平呈正相关关系,与HBs Ag和ALT水平无相关性,HBs Ag水平作为HBV-DNA的替代方法仍需进一步验证。Objective：To explore the correlation between hepatitis B virus（HBV）-DNA load and routine serum markers in patients with HBV infection. Methods：The serum HBV-DNA,hepatitis B virus surface antigen（HBsAg）,hepatitis B virus e antigen（HBeAg） and alanine aminotransferase （ ALT ） in 214 patients with HBV infection were detected using TaqMan real time polymerase chain reaction,chemiluminescence and rate assay. The relationship between the markers were analyzed. Results：Among the 214 patients,the positive rates of HBV-DNA and HBeAg were 57. 48% and 28. 04%,respectively. The HBV-DNA load was not related to HBsAg and ALT（P＆gt;0. 05）,and was closely related to the HBeAg level（P＆lt;0. 01）. The levels of HBeAg and ALT in positive HBV-DNA group were significantly higher than those in negative HBV-DNA group（P＆lt;0. 01）. Conclusions：The HBV-DNA load is positive correlation with the HBeAg level,and is not correlation with HBsAg and ALT. The HBsAg level standing for HBsAg still nends be further validated.

关 键 词：乙型肝炎病毒 乙肝表面抗原 乙肝E抗原 荧光定量PCR 

分 类 号：R373.21[医药卫生—病原生物学]