Effects of cerous nitrate on growth and tanshinone production in salvia miltiorrhiza hairy roots  

作　　者：韩名宇 郭万里 梁宗锁 杨东风 闫希军 朱永宏 刘岩 

机构地区：[1]College of Life Science, Zhejiang Sci-Tech University [2]Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province [3]Tasly R&D Institute, Tasly Holding Group Co., Ltd.

出　　处：《Journal of Rare Earths》2015年第11期1228-1235,共8页稀土学报（英文版）

基　　金：supported by National Natural Science Foundation of China(81373908,81403033);Public Project of Technological Application in Zhejiang Province(2014C32108);China Postdoctoral Science Foundation Funded Project(2014M551771);Zhejiang Provincial Top Key Discipline of Biology

摘　　要：Salvia miltiorrhiza, a popular traditional Chinese medicine, is widely used for treatments in cardiotonic disease. Tanshinones are a group of bioactive ingredients in S. miltiorrhiza. In this study, Ce^3＋ was used as an elicitor to enhance tanshinones production in S. miltiorrhiza hairy roots. The results showed that contents of dihydrotanshinone I（DTI） and cryptotanshinone（CT） were significantly enhanced by 50 μmol/L Ce^3＋, and reached to 0.875 mg/g and 0.271 mg/g, respectively. However, tanshinone II A（TIIA） and tanshinone I（TI） contents were significantly decreased to 59% and 62% of the control. Simultaneously, expressions of genes（HMGR, DXR, DXS1, DXS2 and GGPPS） involved in tanshinone biosynthesis were upregulated by Ce^3＋. Responses of DXS1, DXS2 and GGPPS to Ce^3＋ treatments were later than HMGR and DXR. We speculated that branch pathways of DTI and CT biosynthesis were probably different from TIIA and TI. This work will help us understand biosynthetic mechanism of tanshinones in plants.Salvia miltiorrhiza, a popular traditional Chinese medicine, is widely used for treatments in cardiotonic disease. Tanshinones are a group of bioactive ingredients in S. miltiorrhiza. In this study, Ce^3＋ was used as an elicitor to enhance tanshinones production in S. miltiorrhiza hairy roots. The results showed that contents of dihydrotanshinone I（DTI） and cryptotanshinone（CT） were significantly enhanced by 50 μmol/L Ce^3＋, and reached to 0.875 mg/g and 0.271 mg/g, respectively. However, tanshinone II A（TIIA） and tanshinone I（TI） contents were significantly decreased to 59% and 62% of the control. Simultaneously, expressions of genes（HMGR, DXR, DXS1, DXS2 and GGPPS） involved in tanshinone biosynthesis were upregulated by Ce^3＋. Responses of DXS1, DXS2 and GGPPS to Ce^3＋ treatments were later than HMGR and DXR. We speculated that branch pathways of DTI and CT biosynthesis were probably different from TIIA and TI. This work will help us understand biosynthetic mechanism of tanshinones in plants.

关 键 词：tanshinones Salvia miltiorrhiza Ce^3＋ secondary metabolism rare earth element 

分 类 号：TQ461[化学工程—制药化工]