硅酸盐可吸收性骨水泥的动物体内生物学评价  

Evaluation of Silicate Absorbable Bone Cement in Animals

作　　者：朱海波[1] 马南[1] 钟务学[1] 

机构地区：[1]上海市徐汇区中心医院骨科,上海200031

出　　处：《实用骨科杂志》2015年第10期902-906,共5页Journal of Practical Orthopaedics

基　　金：上海市自然科学基金资助(11ZR1433900)

摘　　要：目的通过动物实验观察一种新型的硅酸盐可吸收性骨水泥的生物相容性、生物降解性和成骨特性。方法选取24只新西兰大白兔,在双侧股骨髁部钻取直径5 mm、高10 mm圆柱形骨缺损,分别置入磷酸钙骨水泥(calcium phosphate cements,CPC,对照组)和磷酸钙-硅酸三钙复合骨水泥(calcium phosphate cements-calcium silicate,CPC-CS,材料组),术后4、8、12周分别处死8只动物,行X线检查和HE染色观察材料在体内的降解和新骨形成情况。结果 4周时材料组和对照组材料无降解,HE染色观察材料-骨组织之间均形成纤维层,8周时材料组材料降解变软,对照组材料降解不明显,对照组和材料组材料-骨之间纤维组织逐渐被新骨取代,材料组材料-骨之间结合面积明显较大,有较多新生骨;12周时,材料组材料降解明显,内部明显发生碎裂,有新生骨长入,对照组材料仅部分降解,对照组材料降解率为32.4%,材料组为45.4%,材料组降解速率显著高于对照组,差异有统计学意义(P<0.01)。结论磷酸钙-硅酸三钙复合骨水泥具备单纯磷酸钙类似的生物相容性,并且在力学强度、表面成骨活性和降解性方面有一定改善,通过进一步的优化有可能成为一种具有很好应用前景的新型生物活性骨修复材料。Objectiv To observe biocompatibility,biodegradability and osteogenesis characteristic of a new kind of silicate bone cement. Methods Bone defects of 5 mm × 10 mm were made at both femoral condyles of 24 rabbits,and calcium phosphate cement（ CPC） or calcium-phosphate-silicate composite bone cement（ CPC-CS） were injected in bone defects of rabbits.We observe the degradation of material in the body and new bone formation through X-ray and HE staining in 4. 8. 12 weeks after operation. Results Without degradation of materials at 4 weeks,the fiber tissues were formed between materials and bones. The CPC-CS materials degradation was more obvious than the CPC materials,the fiber tissues between materials and bones gradually were replaced by new bone tissues,more new bone was found in CPC-CS group at 8 weeks. At 12 weeks,the CPC-CS materials degradation was more obvious than the CPC materials,the CPC group degradation rate was 32. 4%,CPC-CS group was 45. 4%,the CPC-CS materials degradation rate was significantly higher than the CPC materials（ P〈0. 01）. Conclusion Calcium-phosphate-silicate composite bone cement has good biocompatibility,mechanical strength,osteogenesis characteristic and degradability. Calcium-phosphate-silicate composite bone cement will be a new bioactive bone repair material.

关 键 词：骨水泥 磷酸钙 硅酸三钙 动物实验 

分 类 号：R687.34[医药卫生—骨科学]