壹期矽肺患者血清8-iso-PGF2α、T-AOC和MDC水平变化  

Serum levels of 8-iso-PGF2α,T-AOC and MDC in stage Ⅰ silicosis patients

作　　者：王冬梅[1] 袁宝军[1] 佟艳艳[1] 李超[1] 邹吉敏[1] 高利常[1] 

机构地区：[1]开滦总医院检验科,河北唐山063000

出　　处：《中国工业医学杂志》2015年第5期326-328,334,共4页Chinese Journal of Industrial Medicine

摘　　要：目的探讨壹期矽肺患者血清中8-异前列腺素F2α(8-iso-PGF2α)、总抗氧化能力(T-AOC)和巨噬细胞衍生趋化因子(MDC)水平变化及意义。方法采用酶联免疫吸附法检测58例壹期矽肺(矽肺组)、35例具有与矽肺组相同接尘条件的健康井下接尘矿工(接尘组)和33例非接尘井上健康人员(对照组)血清中8-iso-PGF2α水平;用Luminex流式荧光技术检测血清MDC水平;化学比色法检测血清T-AOC水平。结果与对照组比较,接尘和矽肺组血清T-AOC水平均明显升高,接尘组MDC水平则明显降低,差异均具有统计学意义(P<0.01);与接尘组比较,矽肺组血清8-iso-PGF2α和T-AOC水平降低,MDC水平升高,差异也具有统计学意义(P<0.05,P<0.01);矽肺并发慢性阻塞性肺疾病(COPD)患者血清T-AOC水平高于单纯矽肺患者(P<0.05);饮酒矽肺患者血清8-iso-PGF2α水平低于非饮酒矽肺患者,差异具有统计学意义(P<0.05);壹期矽肺患者血清T-AOC与MDC呈负相关(r=-0.247,P<0.05)。结论壹期矽肺患者血清8-iso-PGF2α、T-AOC及MDC水平异常,并与矽肺发生发展有关;饮酒可影响壹期矽肺患者血清8-iso-PGF2α水平。Objective To explore the changes and the significance of serum levels of 8-iso-prostaglandin F2α （8-iso- PGF2α）, total antioxidant capacity （T-AOC） and macrophage-derived chemokine （MDC） in stage I silicosis patients. Methods The levels of serum 8-iso-PGF2ct in fifty-eight stage I silicosis patients （silicosis group）, 35 healthy miners （dust-contacted group） and 33 cases of healthy non underground workers （control group） were detected by enzyme-linked im- munosorbent assays, meanwhile, the MDC and T-AOC levels were also detected by Luminex fluorescence technique and chemis- try colorimentric method, respectively. Results Comparing with control group, the serum T-AOC level in dust-contacted group and silicosis group was significantly increased, the serum MDC level in dust-contacted group was significantly reduced （all P〈 0. O1 ） ; the serum levels of 8-iso-PGF2α and T-AOC were significantly lower and the serum levels of MDC in silicosis group was significantly higher than those of dust-contacted group （ P〈0. 05, P〈0.01 ） and there was a negative correlation between T-AOC and MDC in stage I silicosis patients （r=-0. 247, P〈0. 05） ; additionally, serum levels of T-AOC in silicosis with COPD was significantly higher than that of simple silicosis （ P〈0. 05 ） ; the levels of 8-iso-PGF2α in drinking silicosis patients were significantly lower than that of non-drinking patients （P〈0. 05）. Conclusion The results suggested that in stage I silicosis pa-tients, serum levels of 8-iso-PGF2α, T-AOC and MDC were abnormal and related to the development of silicosis ; and drinking might affect the serum level of 8-iso-PGF2α in silicosis patients.

关 键 词：矽肺 8-异前列腺素F2α(8-iso-PGF2α) 总抗氧化能力(T-AOC) 巨噬细胞衍生趋化因子(MDC) 

分 类 号：R135.2[医药卫生—劳动卫生]