18β-甘草次酸对变应性鼻炎大鼠鼻黏膜中水通道蛋白1和5表达的影响  

Effect of 18β-glycyrrhetinic Acid on Aquaporin 1,5 Expression in the Nasal Mucosa of Rats with Allergic Rhinitis

作　　者：黎桥[1] 席克虎[1] 桂岩[1] 王有虎[1] 张福宏[1] 陈小婉[1] 江英[1] 侯赟[1] 张小兵[1] 

机构地区：[1]兰州大学附属第一医院耳鼻咽喉头颈外科,兰州730000

出　　处：《中国医科大学学报》2015年第11期961-965,共5页Journal of China Medical University

基　　金：国家自然科学基金(81160449);甘肃省卫生厅行业科技计划(GSWST2011-06);甘肃省中医药科学技术研究课题(GZK-2011-22)

摘　　要：目的观察18β-甘草次酸对变应性鼻炎大鼠鼻黏膜水通道蛋白(AQP)表达的影响并探讨其意义。方法将60只Wistar大鼠随机均分为18β-甘草次酸组、氯雷他定组、模型组和正常对照组,18β-甘草次酸组、氯雷他定组及模型组大鼠经卵清蛋白(OVA)致敏建立变应性鼻炎疾病模型,造模成功后分别给药干预,于给药2、4、6、8、10周时,应用免疫组织化学方法(SP法)检测各组鼻黏膜中AQP1和AQP5的表达并进行比较。结果 AQP1和AQP5在各组大鼠鼻黏膜中分布相同,AQP1主要表达于上毛细血管、血窦内皮细胞及成纤维细胞。AQP5强表达于腺上皮、导管上皮及黏膜上皮细胞的胞膜及胞质。免疫组化染色统计学分析显示,AQP1和AQP5在模型组的表达量显著高于正常对照组(P<0.05),在18β-甘草次酸组及氯雷他定组大鼠鼻黏膜中的表达量均随给药干预的时间延长逐渐下降,并接近于正常对照组。结论 18β-甘草次酸可能通过下调AQP1和AQP5的表达缓解变应性鼻炎挠鼻、喷嚏及流涕等症状。Objective To investigate the influence and its significance of 18β-glycyrrhetinic acid on the expression of aquaqporin 1,5 （AQP1,5） in rat nasal mucosa with allergic rhinitis （AR）. Methods Sixty Wistar rats were randomly divided into four groups ： model control group, loratadine group, 18β-glycyrrhetinic acid group and normal control group. Rats in model control group, lomtadine group and 18β-glycyrrhetinic acid group were first induced for allergic rhinitis by dripping ovalbumin, and then were treated respectively. After 2 weeks, 4 weeks, 6 weeks, 8 weeks and 10 weeks, the expression of AQP1,5 in the nasal mucosa of each group was investigated and compared by immunohisto chemical technique. Results The distribution of AQP1,5 was consistent in all groups. AQP 1 expressed mainly in capillary and blood sinus endothelial cells, and fibrocytes. AQP5 expressed intensively in the membrane and cytoplasm of the epithelium in the glands, cilia, and ductal epithelial area. The statistical analysis of im- munohistochemical staining showed that the quantity of AQP1,5 in model group was significantly higher than that in normal group （P 〈 0.05）, and with the extension of drug intervention time, the expression of AQP1,5 in loratadine group and 18β-glycyrrhetinic acid group were gradually de- creased and almost equivalent to normal group. Conclusion 18β-glycyrrhetinic may relieve symptoms of AR by down-regulating the expression of AQP1,5.

关 键 词：18Β-甘草次酸 水通道蛋白1 水通道蛋白5 变应性鼻炎 免疫组织化学 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]