微柱对细胞核形态影响的研究  

Studys on Effects of Micropillars on Cell Nucleus Shapes

作　　者：安燕飞[1,2] 王进义[2] 

机构地区：[1]长治医学院生物学教研室,046000 [2]西北农林科技大学动物医学院

出　　处：《长治医学院学报》2015年第5期333-336,共4页Journal of Changzhi Medical College

摘　　要：目的:探讨微柱对3种细胞细胞核形态的影响。方法:在高度10μm,直径18μm,间距15μm的PDMS微柱上分别培养3种细胞(表皮细胞、内皮细胞和成纤维细胞),24h后对其细胞核形态参数包括平均面积、周长、纵横比以及圆度值进行测量,并与其在平面上的相应值进行比较分析。结果:微柱上表皮细胞和内皮细胞的细胞核变形明显,核平均面积较平面上小很多,出现了弯月形和哑铃形;而成纤维细胞核则变形较小,仍为椭圆形,只是纵横比增大。结论:本实验采用的微柱尺度会使得3种细胞有不同程度的细胞核变形。Objective：Effects of micropillar on cell nucleus shapes of three different kinds of cells was discussed.Methods：First, these three different kinds of cells（epithelial cell,fibroblast and endothelial cell）were cultured on an array of PDMS micropillars which were 10 μm in height,18 μm in diameter and 15 μm in edge to edge spacing.Then,a quantitative and comparative analysis of the cell nucleus shapes was given by measuring the average area,aspect ratio and their circularity of the cells both on the micropillars and on the flat substrate.Results：Distinct nuclear deformation such as crescent,and dumbbell was observed of epithelial cells and endothelial cells on the micropillar substrate and their nuclear average areas were much smaller than that of the the cells on the flat substrate,when nucleus shape of fibroblasts was still in oval but just with a larger aspect ratio.Conclusion：Different degree of nuclear deformation would be induced by the micropillars that we used in this paper.

关 键 词：微柱 表皮细胞 内皮细胞 成纤维细胞 细胞核形态 

分 类 号：R318[医药卫生—生物医学工程]