人参皂苷Rg1通过NF-κB通路减轻大鼠心肌缺血再灌注损伤  

Ginsenoside Rg1 attenuates myocardial ischemia reperfusion injury through NF-κB signaling pathway

作　　者：贺欣[1] 张英杰[1] 

机构地区：[1]辽宁医学院附属第一医院心内科,锦州121000

出　　处：《中药药理与临床》2015年第4期16-19,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：辽宁省自然科学基金;基金号为:2013022002

摘　　要：目的:探讨人参皂苷Rg1对心肌缺血再灌注损伤的保护作用及其可能的机制。方法:50只SD大鼠随机分为1假手术组、②模型组、③人参皂苷Rg1 1mg/kg、④人参皂苷Rg1 5mg/kg⑤人参皂苷Rg1 10 mg/kg。采用冠状动脉结扎法建立大鼠心肌缺血再灌注损伤模型。HE染色观察心肌形态学变化;氯化硝基四氮唑蓝(NBT)法测定心肌梗死面积;TUNEL法测心肌细胞凋亡;酶联免疫吸附法测定血清中TNF-α,IL-1β含量;电泳法测定心肌组织中p65,IκB表达。结果:10 mg/kg人参皂苷Rg1预处理可改善缺血再灌注损伤引起心肌组织的形态学变化,降低心肌梗死面积,减轻心肌细胞凋亡程度,降低TNF-α,IL-1β含量和p65蛋白表达,增加IκB蛋白表达。结论:人参皂苷Rg1对缺血心肌再灌注损伤具有一定的保护作用,其机制可能通过下调NF-κB通路,降低炎症反应有关。Object： The aim of the present study was to investigate the protective effect of ginsenoside Rg1 on myocardial ischemia reperfusion injury and the underlying mechanism. Methods： 50 SD rats were randomly divided into 5 groups： ①sham operation group（ Sham group）,②myocardial ischemia-reperfusion（ MI / R） group,③ginsenoside Rg1（ 1 mg / kg） group ④ginsenoside Rg1（ 5 mg / kg） group,⑤ginsenoside Rg1（ 10 mg / kg）. Ischemia was produced by ligating the left anterior descending coronary artery（ LAD）. Myocardial infarct size was determined by NBT staining technique and a digital imaging system; myocardial pathological changes were observed by HE staining; Cardiomyocytes apoptosis were determined by TUNEL staining; TNF- and IL-1β were determined by ELISA; p65 and IκB protein expression was determined by Western blot. Results： Pretreatment of ginsenoside Rg1（ 10 mg / kg） before MI / R could attenuate the morphological change of myocardial tissue,decreased the infracted sized,cardiomyocytes apoptosis,the content of TNF- and IL-1β,p65 protein content,and increased the IκB protein expression. Conclusion： Ginsenoside Rg1 pretreatment could attenuate MI / R injury through inhibition of the NF-κB signaling pathway,which led to reduced inflammatory reaction.

关 键 词：心肌缺血再灌注 人参皂苷RG1 炎症 NF-ΚB 

分 类 号：R285.5[医药卫生—中药学]